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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorMorrell, Jane Margaret
dc.contributor.authorNúñez González, A.
dc.contributor.authorCrespo Félez, I.
dc.contributor.authorMartínez Martínez, Sonia 
dc.contributor.authorMartínez Alborcia, María J.
dc.contributor.authorFernández Alegre, Estela
dc.contributor.authorDomínguez Fernández de Tejerina, Juan Carlos 
dc.contributor.authorGutiérrez Martín, César Bernardo 
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2019-03-01
dc.date.accessioned2019-05-21T11:24:08Z
dc.date.available2019-05-21T11:24:08Z
dc.date.issued2019-05-21
dc.identifier.citationTheriogenology, 2019, vol. 126es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0093691X18308331#!es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10808
dc.descriptionP. 272-278es_ES
dc.description.abstractAntibiotics are added to semen extenders when preparing commercial semen doses for artificial insemination according to national and international guidelines. However, this addition of antibiotics represents non-therapeutic usage and could be contributing to the development of antibiotic resistance. Colloid centrifugation was shown to reduce the load of bacteria present in boar semen and was capable of removing all bacteria if performed directly after semen collection, albeit with some loss of spermatozoa. The present experiment was conducted with a low density colloid to investigate whether it was possible to separate all of the spermatozoa from seminal plasma i.e. without selection for robust spermatozoa, or whether this would have a detrimental effect on sperm quality. Ejaculates from nine boars were extended in Beltsville Thawing Solution without antibiotics and were transported to the laboratory for Single Layer Centrifugation (SLC) on modified Porcicoll i.e. at a low density (S). A further modification was that a sterile inner tube was included inside some of the 50 mL centrifuge tubes to facilitate harvesting of the sperm pellet (M). Aliquots of all samples (control, S and M) were cultured for bacterial quantification and identification using standard microbiological methods. Sperm quality was evaluated daily. Three of the C and M samples and five of the S samples did not contain any bacteria. Mean bacterial counts for the remaining samples (colony forming units/mL) were as follows: C 259 ± 216; S 30 ± 22; M 33 ± 15 (P < 0.01). Citrobacter spp., Staphylococcus simulans, Klebsiella variicola, Escherichia coli, Myroides odoratimimus, Proteus spp. and Enterococcus faecalis were identified in the control samples. There were marginal differences in sperm quality among treatments, with sperm velocity and linearity being higher in S and M samples than in C at all time points. However, sperm viability, capacitation and acrosome status were marginally better in controls than in S or M on day 0, but these differences disappeared during storage. Conclusions: centrifugation through a low density colloid can remove or reduce bacterial contamination in boar ejaculates without using antibiotics. Furthermore, it is possible to collect boar ejaculates without bacterial contamination by paying strict attention to hygiene.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherJabalíeses_ES
dc.subject.otherSemenes_ES
dc.subject.otherCentrifugaciónes_ES
dc.subject.otherColoideses_ES
dc.titleRemoval of bacteria from boar semen using a low-density colloides_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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