Cleaved PARP‐1, an Apoptotic Marker, can be Detected in Ram Spermatozoa

DSpace/Manakin Repository

Cleaved PARP‐1, an Apoptotic Marker, can be Detected in Ram Spermatozoa

Show full item record

Title: Cleaved PARP‐1, an Apoptotic Marker, can be Detected in Ram Spermatozoa
Author: Casao Gascón, Adriana;Mata Campuzano, María;Ordás, L.;Cebrián Pérez, José A.;Muiño Blanco, María Teresa;Martínez Pastor, Felipe
xmlui.dri2xhtml.METS-1.0.item-contributor: Facultad de Ciencias Biologicas y Ambientales
xmlui.dri2xhtml.METS-1.0.item-area: Biologia Celular
Abstract: The presence of apoptotic features in spermatozoa has been related to lower quality and functional impairment. Members of the poly‐ADP‐ribose polymerases (PARP) familyare involved in both DNA repair and apoptosis, playing important roles in spermatogenesis. Poly‐ADP‐ribose polymerase can be cleaved by caspases, and the presence of its cleavage product (cPARP) in spermatozoa has been related to chromatin remodelling during spermatogenesis and to the activation of apoptotic pathways. There are no reports on immunodetection of cPARP in ram spermatozoa; thus, we have tested a commercially available antibody for this purpose. cPARP was microscopically detected in the acrosomal ridge of some spermatozoa (indirect immunofluorescence). A preliminary study was carried out by flow cytometry (direct immunofluorescence, FITC). Ram semen was extended in TALP and incubated for 4 h with apoptosis inducers staurosporine (10 μm) or betulinic acid (200 μm). Both inducers and incubation caused a significant increase in cPARP spermatozoa (0 h, control: 21.4±3.3%, inducers: 44.3±1.4%; 4 h, control: 44.3±2.4%, inducers: 53.3±1.4%). In a second experiment, we compared the sperm fractions after density gradient separation (pellet and interface). The pellet yielded a slightly lower proportion of cPARP spermatozoa (28.5±1.2% vs 36.2±2.0% in the interface; p < 0.001), and a 12‐h incubation increased cPARP similarly in both fractions (p < 0.001). cPARP seems to be an early marker of apoptosis in ram semen, although its presence in untreated samples was weakly related to worse quality (pellet/interface). We suggest to study the relationship of PARP and cPARP levels with between‐male differences on sperm fertility.
xmlui.dri2xhtml.METS-1.0.item-desfisica: P. 688-691
xmlui.dri2xhtml.METS-1.0.item-peerreviewed: SI
Publisher: John Wiley & Sons
xmlui.dri2xhtml.METS-1.0.item-citation: Reproduction in Domestic Animals, 2015, vol. 50, n. 4
URI: http://hdl.handle.net/10612/10829
Date: 2015-08
xmlui.dri2xhtml.METS-1.0.item-tipo: info:eu-repo/semantics/article
Subject: Veterinaria
xmlui.dri2xhtml.METS-1.0.item-palclave: Polimerasas
Esperma
Ganado ovino
Exportar referencia a Refworks:


Files in this item

Files Size Format View
78 The Apoptoti ... mestAnim_050_0688_2015.pdf 245.4Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record

Search DSpace


Advanced Search

Browse

My Account

Statistics