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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorCasao Gascón, Adriana
dc.contributor.authorMata Campuzano, María
dc.contributor.authorOrdás, L.
dc.contributor.authorCebrián Pérez, José A.
dc.contributor.authorMuiño Blanco, María Teresa
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2015-08
dc.date.accessioned2019-05-24T09:55:59Z
dc.date.available2019-05-24T09:55:59Z
dc.date.issued2019-05-24
dc.identifier.citationReproduction in Domestic Animals, 2015, vol. 50, n. 4es_ES
dc.identifier.otherhttps://onlinelibrary.wiley.com/doi/full/10.1111/rda.12549es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10829
dc.descriptionP. 688-691es_ES
dc.description.abstractThe presence of apoptotic features in spermatozoa has been related to lower quality and functional impairment. Members of the poly‐ADP‐ribose polymerases (PARP) familyare involved in both DNA repair and apoptosis, playing important roles in spermatogenesis. Poly‐ADP‐ribose polymerase can be cleaved by caspases, and the presence of its cleavage product (cPARP) in spermatozoa has been related to chromatin remodelling during spermatogenesis and to the activation of apoptotic pathways. There are no reports on immunodetection of cPARP in ram spermatozoa; thus, we have tested a commercially available antibody for this purpose. cPARP was microscopically detected in the acrosomal ridge of some spermatozoa (indirect immunofluorescence). A preliminary study was carried out by flow cytometry (direct immunofluorescence, FITC). Ram semen was extended in TALP and incubated for 4 h with apoptosis inducers staurosporine (10 μm) or betulinic acid (200 μm). Both inducers and incubation caused a significant increase in cPARP spermatozoa (0 h, control: 21.4±3.3%, inducers: 44.3±1.4%; 4 h, control: 44.3±2.4%, inducers: 53.3±1.4%). In a second experiment, we compared the sperm fractions after density gradient separation (pellet and interface). The pellet yielded a slightly lower proportion of cPARP spermatozoa (28.5±1.2% vs 36.2±2.0% in the interface; p < 0.001), and a 12‐h incubation increased cPARP similarly in both fractions (p < 0.001). cPARP seems to be an early marker of apoptosis in ram semen, although its presence in untreated samples was weakly related to worse quality (pellet/interface). We suggest to study the relationship of PARP and cPARP levels with between‐male differences on sperm fertility.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherPolimerasases_ES
dc.subject.otherEspermaes_ES
dc.subject.otherGanado ovinoes_ES
dc.titleCleaved PARP‐1, an Apoptotic Marker, can be Detected in Ram Spermatozoaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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