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    Título
    A genome-wide identification and comparative analysis of the lentil MLO genes
    Autor
    Polanco de la Puente, Carlos GasparAutoridad BuleriaORCID
    Sáenz De Miera Carnicer, Luis Enrique
    Bett, Kirstin
    Pérez de la Vega, MarcelinoAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Genetica
    Título de la revista
    PLOS ONE
    Número de la revista
    3
    Datos de la obra
    PLoS ONE 13(3): e0194945.
    Editor
    PLoS
    Fecha
    2018
    Abstract
    Powdery mildew is a widespread fungal plant disease that can cause significant losses in many crops. Some MLO genes (Mildew resistance locus O) have proved to confer a durable resistance to powdery mildew in several species. Resistance granted by the MLO gene family members has prompted an increasing interest in characterizing these genes and implementing their use in plant breeding. Lentil (Lens culinaris Medik.) is a widely grown food legume almost exclusively consumed as dry seed with an average world production of 4.5 million tons. Powdery mildew causes severe losses on certain lentil cultivars under particular environmental conditions. Data mining of the lentil CDC Redberry draft genome allowed to identify up to 15 gene sequences with homology to known MLO genes, designated as LcMLOs. Further characterization of these gene sequences and their deduced protein sequences demonstrated conformity with key MLO protein characteristics such as the presence of transmembrane and calmodulin binding domains, as well as that of other conserved motifs. Phylogenetic and other comparative analyses revealed that LcMLO1 and LcMLO3 are the most likely gene orthologs related to powdery mildew response in other species, sharing a high similarity with other known resistance genes of dicot species, such as pea PsMLO1 and Medicago truncatula MtMLO1 and MtMLO3. Sets of primers were designed as tools to PCR amplify the genomic sequences of LcMLO1 and LcMLO3, also to screen lentil germplasm in search of resistance mutants. Primers were used to obtain the complete sequences of these two genes in all of the six wild lentil relatives. Respective to each gene, all Lens sequences shared a high similarity. Likewise, we used these primers to screen a working collection of 58 cultivated and 23 wild lentil accessions in search of length polymorphisms present in these two genes. All these data widen the insights on this gene family and can be useful for breeding programs in lentil and close related species.
    Materia
    Genética
    Palabras clave
    Genoma
    Peer review
    SI
    ID proyecto
    • AGL2013- 44714-R, FP7 613551 (LEGumes for the Agriculture of Tomorrow; LEGATO), Junta de Castilla y León (GR113)
    URI
    http://hdl.handle.net/10612/11116
    DOI
    10.1371/journal.pone.0194945
    Versión del editor
    https://doi.org/10.1371/journal.pone.0194945
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