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dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorFernández Gómez, Paula 
dc.contributor.authorCobo Díaz, José Francisco 
dc.contributor.authorSousa Oliveira, Marcia Patricia de 
dc.contributor.authorGonzález Raurich, María Montserrat 
dc.contributor.authorÁlvarez Ordóñez, Avelino 
dc.contributor.authorPrieto Maradona, Miguel 
dc.contributor.authorWalsh, James L.
dc.contributor.authorSivertsvik, Morten
dc.contributor.authorNoriega Fernández, Estefanía
dc.contributor.authorLópez Fernández, María Mercedes 
dc.contributor.otherTecnologia de los Alimentoses_ES
dc.identifier.citationFernández-Gómez, P., Cobo-Díaz, J. F., Oliveira, M., González-Raurich, M., Alvarez-Ordóñez, A., Prieto, M., Walsh, J. L., Sivertsvik, M., Noriega-Fernández, E., & López, M. (2023). Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells. Food Microbiology, 113(104252), 104252.
dc.description.abstract[EN] Plasma-Activated Water (PAW) was generated from tap water using a surface dielectric barrier discharge at different discharge power (26 and 36 W) and activation time (5 and 30 min). The inactivation of a three-strain Listeria monocytogenes cocktail in planktonic and biofilm state was evaluated. PAW generated at 36 W-30 min showed the lowest pH and the highest hydrogen peroxide, nitrates, nitrites contents and effectiveness against cells on planktonic state, resulting in 4.6 log reductions after a 15-min treatment. Although the antimicrobial activity in biofilms formed on stainless steel and on polystyrene was lower, increasing the exposure time to 30 min allowed an inactivation >4.5 log cycles. The mechanisms of action of PAW were investigated using chemical solutions that mimic its physico-chemical characteristics and also RNA-seq analysis. The main transcriptomic changes affected carbon metabolism, virulence and general stress response genes, with several overexpressed genes belonging to the cobalamin-dependent gene cluster.es_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectTecnología de los alimentoses_ES
dc.subject.otherPlasma-activated wateres_ES
dc.subject.otherAntimicrobial activityes_ES
dc.subject.otherListeria monocytogeneses_ES
dc.titleSusceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cellses_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-113658RB-C22/ES/NUEVAS ESTRATEGIAS BASADAS EN LA TECNOLOGIA DE PLASMA PARA REDUCIR LA FORMACION DE BIOFILMS EN LA INDUSTRIA LACTEAes_ES
dc.journal.titleFood Microbiologyes_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional