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Título
Stress decreases spermatozoa quality and induces molecular alterations in zebrafish progeny
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
BMC Biology
Cita Bibliográfica
Valcarce, D.G., Riesco, M.F., Cuesta-Martín, L., Esteve-Codina, A., Martínez-Vájuez, J.M., Robles, V. (2023). Stress decreases spermatozoa quality and induces molecular alterations in zebrafish progeny. 21, 70 (2023). https://doi.org/10.1186/s12915-023-01570-w
Editorial
Springer Nature
Fecha
2023
Resumen
[EN] Background Chronic stress can produce a severe negative impact on health not only in the exposed individuals
but also in their offspring. Indeed, chronic stress may be contributing to the current worldwide scenario of increasing
infertility and decreasing gamete quality in human populations. Here, we evaluate the effect of chronic stress
on behavior and male reproductive parameters in zebrafish. Our goal is to provide information on the impact that
chronic stress has at molecular, histological, and physiological level in a vertebrate model species.
Results We evaluated the effects of a 21-day chronic stress protocol covering around three full waves of spermatogenesis
in Danio rerio adult males. The induction of chronic stress produced anxiety-like behavior in stressed males as
assessed by a novel tank test. At a molecular level, the induction of chronic stress consistently resulted in the overexpression
of two genes related to endoplasmic reticulum (ER) stress in the brain. Gene set enrichment analysis (GSEA)
of testes suggested a dysregulation of the nonsense-mediated decay (NMD) pathway, which was also confirmed on
qPCR analysis. Histological analysis of the testicle did not show significant differences in terms of the relative proportions
of each germ-cell type; however, the quality of sperm from stressed males was compromised in terms of motility.
RNA-seq analysis in stress-derived larval progenies revealed molecular alterations, including those predicted to
affect translation initiation, DNA repair, cell cycle control, and response to stress.
Conclusions Induction of chronic stress during a few cycles of spermatogenesis in the vertebrate zebrafish model
affects behavior, gonadal gene expression, final gamete quality, and progeny. The NMD surveillance pathway (a key
cellular mechanism that regulates the stability of both normal and mutant transcripts) is severely affected in the testes
by chronic stress and therefore the control and regulation of RNAs during spermatogenesis may be affected altering
the molecular status in the progeny.
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ID proyecto
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/ PID2019-108509RB-I00/ES/ EL ESTRES EN LA REPRODUCCION DE PECES: TRANSMISION DE LOS EFECTOS NEGATIVOS A LA PROGENIE Y DESARROLLO DE NUEVAS TECNOLOGIAS PARA REVERTIRLOS
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