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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Rodríguez García, Antonio | |
dc.contributor.author | Fuente, Álvaro de la | |
dc.contributor.author | Pérez Redondo, Rosario | |
dc.contributor.author | Martín Martín, Juan Francisco | |
dc.contributor.author | Liras Padín, Paloma | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2000-04-14 | |
dc.date.accessioned | 2024-01-22T13:12:53Z | |
dc.date.available | 2024-01-22T13:12:53Z | |
dc.identifier.citation | Rodríguez-García, A., De La Fuente, A., Pérez-Redondo, R., Martín, J. F. & Liras, P. (2000). Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus. Journal of Molecular Microbiology and Biotechnology, 2(4), 543-550 | es_ES |
dc.identifier.issn | 1464-1801 | |
dc.identifier.other | https://www.caister.com/backlist/jmmb/tocs/v2n4toc.html | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/17708 | |
dc.description | Revista continuada por Microbial Physiology | es_ES |
dc.description.abstract | [EN] A cluster of genes argCJBDRGH containing most of the arginine biosynthesis genes has been found in Streptomyces clavuligerus after sequencing a 8.3 kb DNA region containing overlapping sequences of two DNA fragments known to contain arginine biosynthesis genes. Subcloning, complementation of E. coli arginine auxotrophic strains and enzymatic assays confirmed the identity of each gene. S1 nuclease mapping studies and Northern hybridization analysis revealed the formation of two large transcripts corresponding to argCJBDR and argGH. The amount of each of these mRNAs is 10 to 44 times higher in a S. clavuligerus argR-disrupted mutant than in the wild type confirming the existence of an ArgR-mediated control of arginine biosynthesis gene expression. A low level constitutive monocistronic transcript of argR was observed in S. clavuligerus cells. Most of the argGH transcript initiating at an adenine 29 nt upstream of the argG initiation codon appears to stop at a termination stem and loop structure present downstream of the argG gene | es_ES |
dc.language | eng | es_ES |
dc.publisher | Karger | es_ES |
dc.subject | Biotecnología | es_ES |
dc.subject.other | Streptomyces clavuligerus | es_ES |
dc.subject.other | S1 nuclease assay | es_ES |
dc.subject.other | Northern hybridization | es_ES |
dc.subject.other | N-acetylglutamate synthetase | es_ES |
dc.subject.other | N-acetylornithine aminotransferase | es_ES |
dc.subject.other | N-acetylornithinase | es_ES |
dc.subject.other | Arginine biosynthesis | es_ES |
dc.subject.other | argCJBDRGH | es_ES |
dc.subject.other | Ornithine N-acetyltransferase | es_ES |
dc.title | Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Plan Nacional de Investigación Científica y Desarrollo Tecnológico/BIO96-0827/ES/Ingeniería metabólica de streptomyces clavuligerus para la eliminación de los mecanismos de control que limitan la biosíntesis del ácido clavulánico y de sus precursores | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.journal.title | Journal of Molecular Microbiology and Biotechnology | es_ES |
dc.volume.number | 2 | es_ES |
dc.issue.number | 4 | es_ES |
dc.page.initial | 543 | es_ES |
dc.page.final | 550 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2414.03 Metabolismo Bacteriano | es_ES |
dc.subject.unesco | 2415.01 Biología Molecular de Microorganismos | es_ES |
dc.description.project | This work was supported by grants from the CICYT (Madrid) Bio96-0827 and by Antibióticos SA, León. We thank H. Kieser (Norwich, U.K.) for providing the S. coelicolor cosmid library. Álvaro de la Fuente and Rosario Pérez-Redondo received fellowships from the PFPI (Madrid) and the University of León, respectively. | es_ES |
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