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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Rodríguez García, Antonio | |
dc.contributor.author | Combes, Patricia | |
dc.contributor.author | Pérez Redondo, Rosario | |
dc.contributor.author | Smith, Matthew C. A. | |
dc.contributor.author | Smith, Margaret C. M. | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2005-05-01 | |
dc.date.accessioned | 2024-01-23T10:04:46Z | |
dc.date.available | 2024-01-23T10:04:46Z | |
dc.identifier.citation | Rodríguez-García, A., Combes, P., Pérez-Redondo, R., Smith, M. C. A., & Smith, M. C. M. (2005). Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces. Nucleic Acids Research, 33(9), Article e87. https://doi.org/10.1093/NAR/GNI086 | es_ES |
dc.identifier.issn | 0305-1048 | |
dc.identifier.other | https://academic.oup.com/nar/article/33/9/e87/2401590 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/17725 | |
dc.description.abstract | [EN] Bacteria in the genus Streptomyces are major producers of antibiotics and other pharmacologically active compounds. Genetic and physiological manipulations of these bacteria are important for new drug discovery and production development. An essential part of any ‘genetic toolkit’ is the availability of regulatable promoters. We have adapted the tetracycline (Tc) repressor/operator (TetR/ tetO ) regulatable system from transposon Tn 10 for use in Streptomyces . The synthetic Tc controllable promoter (tcp), tcp830 , was active in a wide range of Streptomyces species, and varying levels of induction were observed after the addition of 1–100 ng/ml of anhydrotetracycline (aTc). Streptomyces coelicolor contained an innate Tc-controllable promoter regulated by a TetR homologue (SCO0253). Both natural and synthetic promoters were active and inducible throughout growth. Using the luxAB genes expressing luciferase as a reporter system, we showed that induction factors of up to 270 could be obtained for tcp830 . The effect of inducers on the growth of S.coelicolor was determined; addition of aTc at concentrations where induction is optimal, i.e. 0.1–1 μg/ml, ranged from no effect on growth rate to a small increase in the lag period compared with cultures with no inducer | es_ES |
dc.language | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.subject | Biotecnología | es_ES |
dc.subject.other | Inducible promoter | es_ES |
dc.subject.other | Streptomyces | es_ES |
dc.subject.other | Tetracycline operator | es_ES |
dc.subject.other | TetR | es_ES |
dc.subject.other | Synthetic promoter | es_ES |
dc.subject.other | Anhydrotetracycline | es_ES |
dc.subject.other | Luciferase reporter | es_ES |
dc.title | Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1093/nar/gni086 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/BBSRC/Investigating Gene Function 1999-2005/IGF12436/UK/A concerted approach to global mutational analysis of gene function in Streptomyces coelicolor | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1362-4962 | |
dc.journal.title | Nucleic Acids Research | es_ES |
dc.volume.number | 33 | es_ES |
dc.issue.number | 9 | es_ES |
dc.page.initial | 87 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2415.01 Biología Molecular de Microorganismos | es_ES |
dc.description.project | The authors acknowledge gifts of plasmids and strains from Prof. Leadlay, Prof. Hillen, Prof. Bujard, Dr Herron and Dr Paget. The authors thank Dr Sumby, Dr Ding and Wael Hussein for the construction of several plasmids and vectors. The authors also thank Prof. Williams for the use of Lucy. This work was funded by the BBSRC. Funding to pay the Open Access publication charges for this article was provided by JISC | es_ES |
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