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dc.contributor | Facultad de Ciencias de la Salud | es_ES |
dc.contributor.author | Cardoza, Rosa E. | |
dc.contributor.author | Velasco, Javier | |
dc.contributor.author | Martín Martín, Juan Francisco | |
dc.contributor.author | Liras Padín, Paloma | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2000-09 | |
dc.date.accessioned | 2024-01-25T09:13:42Z | |
dc.date.available | 2024-01-25T09:13:42Z | |
dc.identifier.citation | Cardoza, R. E., Velasco, J., Martín, J. F. M., & Liras, P. (2000). A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans. Applied Microbiology and Biotechnology, 54(3), 406-412. https://doi.org/10.1007/s002530000391 | es_ES |
dc.identifier.issn | 0175-7598 | |
dc.identifier.uri | https://hdl.handle.net/10612/17798 | |
dc.description.abstract | [EN] Two protein bands with strong esterase activity are present in broths of Nocardia lactamdurans MA4213 cultures. One of them shows cephalosporin C acetylhydrolase (CAH) activity. This activity is maximal at 48 h of growth and shows a pattern of regulation slightly different from that of cephamycin production in medium supplemented with glucose (166 mM), glycerol (326 mM) or ammonium chloride (60 mM). The CAH activity was purified to homogeneity by DEAE-Sepharose ion-exchange, Sephadex G-75 gel filtration, and phenyl-Sepharose hydrophobic interaction chromatography. It showed a molecular mass of 72,100 Da. The N-terminus of the protein was determined and showed the amino acid sequence GGAAPGGPGAHPLWLPAGKD. The enzyme showed K(m) values of 7.0 mM and 8.3 mM for cephalosporin C and 7-aminocephalosporanic acid respectively but was not active on cephamycin C. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Springer Link | es_ES |
dc.subject | Biología | es_ES |
dc.subject.other | Cephalosporin C acetylhydrolase | es_ES |
dc.subject.other | Nocardia lactamdurans | es_ES |
dc.subject.other | Deacetylcephalosporin c | es_ES |
dc.subject.other | Beta-lactam antibiotics | es_ES |
dc.title | A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1007/S002530000391 | |
dc.description.peerreviewed | SI | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/closedAccess | es_ES |
dc.journal.title | Applied Microbiology and Biotechnology | es_ES |
dc.volume.number | 54 | es_ES |
dc.issue.number | 3 | es_ES |
dc.page.initial | 406 | es_ES |
dc.page.final | 412 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2414 Microbiología | es_ES |
dc.subject.unesco | 2414.01 Antibióticos | es_ES |
dc.subject.unesco | 2414.07 Metabolismo Microbiano | es_ES |
dc.subject.unesco | 2403 Bioquímica | es_ES |
dc.description.project | This research was supported by a grant BIO97-0289-C02-02 from the Comisión Interministerial de Ciencia y Tecnología (Madrid, Spain). Rosa E. Cardoza received a fellowship from the Dirección General de Asuntos del Personal Académico de la Universidad Nacional Autónoma de Mejico (Mejico, DF). Cephamycin C and the strain N. lactamdurans M4213 were gifts from Merck, Sharp & Dohme (Virginia, USA). Cephalosporin C, deacetylcephalosporin C, 7-aminocephalosporanic acid and 7-am-inodeacetylcephalosporanic acid were obtained from Antibioticos, SA (León, Spain). | es_ES |
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