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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorMartín, María Teresa
dc.contributor.authorCobos Román, Rebeca 
dc.contributor.authorMartín, Laura
dc.contributor.authorLópez Enríquez, Lorena
dc.contributor.otherMicrobiologiaes_ES
dc.date2012-05-05
dc.date.accessioned2024-01-26T09:18:49Z
dc.date.available2024-01-26T09:18:49Z
dc.identifier.citationMartín, M. T., Cobos, R., Martín, L., & López-Enríquez, L. (2012). Real-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum. Applied and Environmental Microbiology, 78(11), 3985-3991. https://doi.org/10.1128/AEM.07360-11es_ES
dc.identifier.issn0099-2240
dc.identifier.otherhttps://journals.asm.org/doi/10.1128/aem.07360-11es_ES
dc.identifier.urihttps://hdl.handle.net/10612/17830
dc.description.abstract[EN] Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for the detection and identification of these pathogens. Real-time PCR based on the amplification of specific sequences is now being used for the identification and quantification of many infective agents. The present work reports real-time PCR protocols for identification of P. chlamydospora and P. aleophilum. Specificity was demonstrated against purified DNA from 60 P. chlamydospora isolates or 61 P. aleophilum isolates, and no amplification was obtained with 54 nontarget DNAs. The limits of detection (i.e., DNA detectable in 95% of reactions) were around 100 fg for P. chlamydospora and 50 fg for P. aleophilum. Detection was specific and sensitive for P. chlamydospora and P. aleophilum. Spores of P. chlamydospora and P. aleophilum were detected without the need for DNA purification. The established protocols detected these fungi in wood samples after DNA purification. P. chlamydospora was detectable without DNA purification and isolation in 67% of reactions. The detection of these pathogens in wood samples has great potential for use in pathogen-free certification schemeses_ES
dc.languageenges_ES
dc.publisherAmerican Society for Microbiology (ASM)es_ES
dc.subjectBiologíaes_ES
dc.subjectBiotecnologíaes_ES
dc.subject.otherGrapevine trunk diseaseses_ES
dc.subject.otherPetri diseasees_ES
dc.subject.otherPhaeomoniella chlamydosporaes_ES
dc.subject.otherqPCR detectiones_ES
dc.subject.otherPhaeoacremonium minimumes_ES
dc.titleReal-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilumes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doidoi:10.1128/AEM.07360-11
dc.description.peerreviewedSIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/Programa Nacional de Contratación e Incorporación/PTA-2003-01-01001/ESes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//INIA RTA04-127/ESes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.essn1098-5336
dc.journal.titleApplied and Environmental Microbiologyes_ES
dc.volume.number78es_ES
dc.issue.number11es_ES
dc.page.initial3985es_ES
dc.page.final3991es_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES
dc.subject.unesco2414.06 Hongoses_ES
dc.subject.unesco2417.09 Fitopatologíaes_ES
dc.description.projectThis research was partly funded by Project INIA RTA04-127. Rebeca Cobos and Laura Martín were supported by an Itacyl-PhD grant and project PTA-2003-01-01001, respectively. Maria Teresa Martín was supported by Contratos Doctores Sistema INIA-CCAA (37, DR07-0161). Marta Hernández provided helpful insight.es_ES


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