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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorGarcía García, Pedro 
dc.contributor.authorVaquero Rodrigo, Francisca 
dc.contributor.authorVences Benito, Francisco Javier 
dc.contributor.authorSáenz de Miera Carnicer, Luis Enrique 
dc.contributor.authorPolanco de la Puente, Carlos Gaspar 
dc.contributor.authorGonzález Cordero, Ana Isabel 
dc.contributor.authorHorres, Ralf
dc.contributor.authorKrezdorn, Nicolas
dc.contributor.authorRotter, Björn
dc.contributor.authorWinter, Peter
dc.contributor.authorPérez de la Vega, Marcelino 
dc.contributor.otherGeneticaes_ES
dc.date2020-02-13
dc.date.accessioned2024-01-26T11:25:50Z
dc.date.available2024-01-26T11:25:50Z
dc.identifier.citationGarcía-García, P., Vaquero, F., Vences, F. J., Sáenz de Miera, L. E., Polanco, C., González, A. I., Horres, R., Krezdorn, N., Rotter, B., Winter, P., & Pérez de la Vega, M. (2020). Transcriptome profiling of lentil in response to Ascochyta lentis infection. Spanish Journal of Agricultural Research, 17(4), Article e0703. https://doi.org/10.5424/sjar/2019174-14982es_ES
dc.identifier.otherhttps://sjar.revistas.csic.es/index.php/sjar/article/view/14982es_ES
dc.identifier.urihttps://hdl.handle.net/10612/17848
dc.descriptionVol. 17 No. 4 (2019) se publicó en febrero de 2020 en la sección Plant breeding, genetics and genetic resourceses_ES
dc.description.abstract[EN] Aim of study: The purpose was to identify some general and genotypic-specific defense responses, in order to obtain a set of candidate genes presumably involved in the resistance. Area of study: The experiment was carried out in León, Spain. Material and methods: We have analyzed the response of three lentil genotypes to Ascochyta lentis (isolate AL 84) at transcriptomic level using the Massive Analysis of cDNA Ends (MACE) technique: the susceptible cultivar 'Lupa', the moderately resistant 'ILL5588' and the resistant wild accession 'BG 16880' (L. culinaris subsp. orientalis). Main results: MACE results yielded a total of 50,935 contigs. The average number of detected contigs in each of the six samples was approximately of 40,000. In response to Ascochyta infection, the jasmonic acid pathway and the lignin biosynthesis were up-regulated in resistant genotypes, while they were down-regulated in the susceptible one. The response to chitin, the salicylic pathway and the auxin response were activated only in the resistant L. c. culinaris genotype, while the giberellin synthesis was only induced in the susceptible L. c. culinaris cv. 'Lupa'. A set of 18 lentil gene sequences putatively involved in the response to the pathogen were validated by RT-qPCR. Research highlights: It can be concluded that in response to the infection by Ascochyta, the lignin biosynthesis and the JA pathway were critical for the resistance, while the giberellin synthesis seems to be related with susceptibility to the pathogenes_ES
dc.languageenges_ES
dc.publisherCSICes_ES
dc.publisherInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)es_ES
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGenéticaes_ES
dc.subject.otherLens culinarises_ES
dc.subject.otherLens orientalises_ES
dc.subject.otherTranscriptomices_ES
dc.subject.otherMACEes_ES
dc.subject.otherMassive Analysis of cDNA Ends techniquees_ES
dc.titleTranscriptome profiling of lentil in response to Ascochyta lentis infectiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.5424/sjar/2019174-14982
dc.description.peerreviewedSIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/AGL2013-44714-R/ES/RESISTENCIA A ESTRESES EN LENTEJA, LENS CULINARIS MEDIK.: DETECCION, ANALISIS GENETICO-GENOMICOS Y MEJORAes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.essn2171-9292
dc.journal.titleSpanish Journal of Agricultural Researches_ES
dc.volume.number17es_ES
dc.issue.number4es_ES
dc.page.initial0703es_ES
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones_ES
dc.subject.unesco2409.92 Genética Molecular de Plantases_ES
dc.subject.unesco2409.02 Ingeniería Genéticaes_ES
dc.description.projectFondos FEDERes_ES
dc.description.projectEspaña. Ministerio de Economía y Competitividades_ES
dc.description.projectEspaña. Junta de Castilla y León. Consejería de Educaciónes_ES


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