Mostrar el registro sencillo del ítem

dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorSola Landa, Alberto
dc.contributor.authorRodríguez García, Antonio 
dc.contributor.authorApel, Alexander Kristian
dc.contributor.authorMartín Martín, Juan Francisco 
dc.contributor.otherMicrobiologiaes_ES
dc.date2008-01-10
dc.date.accessioned2024-01-30T07:49:59Z
dc.date.available2024-01-30T07:49:59Z
dc.identifier.citationSola-Landa, A., Rodríguez-García, A., Apel, A. K., & Martín, J. F. (2008). Target genes and structure of the direct repeats in the DNA-binding sequences of the response regulator PhoP in Streptomyces coelicolor. Nucleic Acids Research, 36(4), 1358-1368. https://doi.org/10.1093/NAR/GKM1150es_ES
dc.identifier.issn0305-1048
dc.identifier.otherhttps://academic.oup.com/nar/article/36/4/1358/2410403?login=truees_ES
dc.identifier.urihttps://hdl.handle.net/10612/17894
dc.description.abstract[EN] Expression of genes belonging to the pho regulon in Streptomyces coelicolor is positively regulated (as shown by comparing the wild-type and a Δ phoP mutant) by binding of the response regulator PhoP to 11-nt direct repeats (DRus). These sequences have been found in over 100 genes of Streptomyces coelicolor ; 20 of them were cloned and the binding of PhoP DBD to most of their promoters has been shown by electrophoretic mobility shift assays. Deletion experiments showed that at least two DRus are required for proper binding of PhoP DBD . Deletion of 1 nt leaving a 10-nt direct repeat reduced drastically binding of PhoP DBD . Three different types of operators have been identified. Complex operators (class III) contain up to six DRus, some of them with poor conservation of the 11-nt consensus sequence, which however were protected by PhoP DBD in footprinting analyses. A cooperative binding of PhoP DBD molecules initiated at conserved core DRus appears to be the mechanism involved in binding of several PhoP DBD monomers to those complex operators. The information theory-based model that incorporates the positive or negative contribution to the binding of PhoP DBD of adjacent sequences has been used to deduce the structure of PHO boxes and the relevance of each DRues_ES
dc.languageenges_ES
dc.publisherOxford University Presses_ES
dc.subjectBiologíaes_ES
dc.subject.otherPhosphate regulationes_ES
dc.subject.otherPhoR-PhoPes_ES
dc.subject.otherPHO boxeses_ES
dc.subject.otherStreptomyceses_ES
dc.subject.otherStreptomyces coelicolores_ES
dc.subject.otherEMSAes_ES
dc.subject.otherDNase footprintinges_ES
dc.titleTarget genes and structure of the direct repeats in the DNA-binding sequences of the response regulator PhoP in Streptomyces coelicolores_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1093/nar/gkm1150
dc.description.peerreviewedSIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EU//LSHM-CT-2004-005224/ACTINOGENes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICYT//BIO2003-01489/ESes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICYT//GEN2003-20245-C09-01/ESes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICYT//BIO2006-14853-C02-01/ESes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.essn1362-4962
dc.journal.titleNucleic Acids Researches_ES
dc.volume.number36es_ES
dc.issue.number4es_ES
dc.page.initial1358es_ES
dc.page.final1368es_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES
dc.subject.unesco2415.01 Biología Molecular de Microorganismoses_ES
dc.subject.unesco2414.02 Fisiología Bacterianaes_ES
dc.description.projectA.K.A. received a fellowship of the FPU Program of the Ministry of Education and Science, Madrid, Spain. We acknowledge the excellent technical help of B. Martín, J. Merino, A. Casenave and B. Aguado. This work was funded by ‘Comisión Interministerial de Ciencia y Tecnología’ (BIO2003-01489, BIO2006-14853-C02-01); ‘Ministerio de Educación, Ciencia y Tecnología’ (GEN2003-20245-C09-01) and ‘European Union’ (ACTINOGEN LSHM-CT-2004-005224). Funding to pay the Open Access publication charges for this article was provided by the ACTINOGEN project.es_ES


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem