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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Santos Beneit, Fernando | |
dc.contributor.author | Rodríguez García, Antonio | |
dc.contributor.author | Sola Landa, Alberto | |
dc.contributor.author | Martín Martín, Juan Francisco | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2009-03-19 | |
dc.date.accessioned | 2024-01-30T09:54:21Z | |
dc.date.available | 2024-01-30T09:54:21Z | |
dc.identifier.citation | Santos-Beneit, F., Rodríguez-García, A., Sola-Landa, A., & Martín, J. F. (2009). Cross-talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription. Molecular Microbiology, 72(1), 53-68. https://doi.org/10.1111/J.1365-2958.2009.06624.X | es_ES |
dc.identifier.issn | 0950-382X | |
dc.identifier.other | https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2009.06624.x | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/17916 | |
dc.description.abstract | [EN] The regulatory proteins AfsR and PhoP control expression of the biosynthesis of actinorhodin and undecylprodigiosin in Streptomyces coelicolor. Electrophoretic mobility shift assays showed that PhoPDBD does not bind directly to the actII-ORF4, redD and atrA promoters, but it binds to the afsS promoter, in a region overlapping with the AfsR operator. DNase I footprinting studies revealed a PhoP protected region of 26 nt (PHO box; two direct repeats of 11 nt) that overlaps with the AfsR binding sequence. Binding experiments indicated a competition between AfsR and PhoP; increasing concentrations of PhoPDBD resulted in the disappearance of the AfsR-DNA complex. Expression studies using the reporter luxAB gene coupled to afsS promoter showed that PhoP downregulates afsS expression probably by a competition with the AfsR activator. Interestingly, AfsR binds to other PhoP-regulated promoters including those of pstS (a component of the phosphate transport system) and phoRP (encoding the two component system itself). Analysis of the AfsR-protected sequences in each of these promoters allowed us to distinguish the AfsR binding sequence from the overlapping PHO box. The reciprocal regulation of the phoRP promoter by AfsR and of afsS by PhoP suggests a fine interplay of these regulators on the control of secondary metabolism | es_ES |
dc.language | eng | es_ES |
dc.publisher | Wiley-Blackwell | es_ES |
dc.subject | Biología | es_ES |
dc.subject.other | Phosphate regulation | es_ES |
dc.subject.other | Antibotics biosynthesis regulation | es_ES |
dc.subject.other | Streptomyces coelicolor | es_ES |
dc.subject.other | PhoR-PhoP | es_ES |
dc.subject.other | AfsR-AfsS | es_ES |
dc.subject.other | PHO boxes | es_ES |
dc.subject.other | DNase footprinting | es_ES |
dc.title | Cross‐talk between two global regulators in Streptomyces: PhoP and AfsR interact in the control of afsS, pstS and phoRP transcription | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1111/j.1365-2958.2009.06624.x | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EU//LSHM-CT-2004-005224/ACTINOGEN | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//GEN2003-20245-C09-01 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//BIO2003-01489/ES | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//BIO2006-14853-C02-01/ES | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//GEN2006-27745-E | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1365-2958 | |
dc.journal.title | Molecular Microbiology | es_ES |
dc.volume.number | 72 | es_ES |
dc.issue.number | 1 | es_ES |
dc.page.initial | 53 | es_ES |
dc.page.final | 68 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/acceptedVersion | es_ES |
dc.subject.unesco | 2415.01 Biología Molecular de Microorganismos | es_ES |
dc.subject.unesco | 2414.02 Fisiología Bacteriana | es_ES |
dc.description.project | This project was supported by grants of the ‘Comisión Interministerial de Ciencia y Tecnología’ (BIO2003-01489, BIO2006-14853-C02-01); Ministry of Education, Science and Technology, Madrid (GEN2003-20245-C09-01); the ERA-NET SySMO Project (GEN2006-27745-E/SYS) and the European Union (ACTINOGEN LSHM-CT-2004-005224). F. Santos-Beneit received a fellowship of the FPI programme (Ministry of Education, Spain). We thank S. Horinouchi for the plasmids containing the afsR gene, M. Bibb for strain S. coelicolor M513 (ΔafsR) and B. Martín, J. Merino, A. Casenave and B. Aguado for excellent technical assistance | es_ES |
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