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Título
Manipulation of bicarbonate concentration in sperm capacitation media improves in vitro fertilisation output in porcine species
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Journal of Animal Science and Biotechnology
Número de la revista
1
Cita Bibliográfica
Soriano-Úbeda, C., Romero-Aguirregomezcorta, J., Matás, C., Visconti, P. E., & García-Vázquez, F. A. (2019). Manipulation of bicarbonate concentration in sperm capacitation media improvesin vitro fertilisation output in porcine species. Journal of Animal Science and Biotechnology, 10(1). https://doi.org/10.1186/S40104-019-0324-Y
Editorial
BMC
Fecha
2019
Resumen
[EN] Background: The in vivo concentration of bicarbonate (HCO3), one of the essential sperm capacitating effectors, varies greatly in the different environments sperm go through from cauda epididymis to the fertilisation site. On the contrary, porcine in vitro sperm capacitation and fertilisation media usually contains a standard concentration of 25 mmol/L, and one of the main problems presented is the unacceptable high incidence of polyspermy. This work hypothesised that by modifying the HCO3 concentration of the medium, the output of in vitro sperm capacitation and fertilisation could be increased. Results: Once exposed to the capacitation medium, the intracellular pH (pHi) of spermatozoa increased immediately even at low concentrations of HCO3, but only extracellular concentrations of and above 15 mmol/L increased the substrates protein kinase A phosphorylation (pPKAs). Although with a significant delay, 15 mmol/L of HCO3 stimulated sperm linear motility and increased other late events in capacitation such as tyrosine phosphorylation (Tyr-P) to levels similar to those obtained with 25 mmol/L. This information allowed the establishment of a new in vitro fertilisation (IVF) system based on the optimization of HCO3 concentration to 15 mmol/L, which led to a 25.3% increment of the viable zygotes (8.6% in the standard system vs. 33.9%). Conclusions: Optimising HCO3 concentrations allows for establishing an IVF method that significantly reduced porcine polyspermy and increased the production of viable zygotes. A concentration of 15 mmol/L of HCO3 in the medium is sufficient to trigger the in vitro sperm capacitation and increase the fertilisation efficiency in porcine
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ID proyecto
- info:eu-repo/grantAgreement/ MINECO / Programa Estatal de I+D+I Orientada a los Retos de la Sociedad /AGL2015-66341-R/ES/ OBTENCION DE ANIMALES SANOS MEDIANTE TECNICAS DE REPRODUCCION ASISTIDA BASADAS EN CONDICIONES FISIOLOGICAS//
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