dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Fregeneda Grandes, Juan Miguel | |
dc.contributor.author | González Palacios, Concepción | |
dc.contributor.author | Pérez Sánchez, Tania | |
dc.contributor.author | Padilla, Daniel | |
dc.contributor.author | Real, Fernando | |
dc.contributor.author | Aller Gancedo, José Miguel | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2023 | |
dc.date.accessioned | 2024-03-05T12:31:32Z | |
dc.date.available | 2024-03-05T12:31:32Z | |
dc.identifier.citation | Fregeneda Grandes, J.-M., González-Palacios, C., Pérez-Sánchez, T., Padilla, D., Real, F., & Aller-Gancedo, J.-M. (2023). Limited Probiotic Effect of Enterococcus gallinarum L1, Vagococcus fluvialis L21 and Lactobacillus plantarum CLFP3 to Protect Rainbow Trout against Saprolegniosis. Animals, 13(5). https://doi.org/10.3390/ANI13050954 | es_ES |
dc.identifier.other | https://www.mdpi.com/2076-2615/13/5/954 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18599 | |
dc.description.abstract | [EN] Previous studies have demonstrated that the strains Enterococcus gallinarum L1, Vagococcus fluvialis L21 and Lactobacillus plantarum CLFP3 are probiotics against vibriosis or lactococosis in sea bass or rainbow trout. In this study, the utility of these bacterial strains in the control of saprolegniosis was evaluated. For this purpose, both in vitro inhibition studies and competition for binding sites against Saprolegnia parasitica and in vivo tests with experimentally infected rainbow trout were carried out. In the in vitro tests, the three isolates showed inhibitory activity upon mycelium growth and cyst germination and reduced the adhesion of cysts to cutaneous mucus; however, this effect depended on the number of bacteria used and the incubation time. In the in vivo test, the bacteria were administered orally at 108 CFU g−1 in the feed or at 106 CFU ml−1 in the tank water for 14 days. None of the three bacteria showed protection against S. parasitica infection either through water or feed, and the cumulative mortality reached 100% within 14 days post infection. The obtained results show that the use of an effective probiotic against a certain disease in a host may not be effective against another pathogen or in another host and that the results obtained in vitro may not always predict the effects when used in vivo. | es_ES |
dc.language | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Biocontrol | es_ES |
dc.subject.other | Probiotics | es_ES |
dc.subject.other | Saprolegniosis | es_ES |
dc.subject.other | Inhibition assays | es_ES |
dc.subject.other | Oncorhynchus mykiss | es_ES |
dc.title | Limited Probiotic Effect of Enterococcus gallinarum L1, Vagococcus fluvialis L21 and Lactobacillus plantarum CLFP3 to Protect Rainbow Trout against Saprolegniosis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.3390/ANI13050954 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-54683-R/ES/NUEVOS PROBIOTICOS Y SUS PRODUCTOS PARA EL CONTROL DE ENFERMEDADES EN ESPECIES RELEVANTES PARA LA ACUICULTURA MARINA Y CONTINENTAL// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2076-2615 | |
dc.journal.title | Animals | es_ES |
dc.volume.number | 13 | es_ES |
dc.issue.number | 5 | es_ES |
dc.page.initial | 954 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.description.project | We are grateful for the assistance provided by the laboratory technician Gloria Fernández–Bayón; the fish farm Los Leoneses in León, which supplied the rainbow trout; the “Servicio Territorial de Medio Ambiente de León” of the “Junta de Castilla y León”, which supplied the brown trout; and Skretting España S.A., which donated some of the feed. | es_ES |
dc.description.project | This study was funded through research project AGL2014-54683-R of the Spanish “Ministerio de Economía y Competitividad” and cofinanced through the European Regional Development Fund (ERDF). C.G.P. was awarded a predoctoral contract by the “Consejería de Educación” of the regional government “Junta de Castilla y León” cofinanced through the European Social Fund. | es_ES |