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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorFernández Díez, Cristina
dc.contributor.authorGonzález Rojo, Silvia
dc.contributor.authorLombó Alonso, Marta 
dc.contributor.authorHerráez Ortega, María Paz 
dc.contributor.otherBiologia Celulares_ES
dc.date2016
dc.date.accessioned2024-03-08T08:25:44Z
dc.date.available2024-03-08T08:25:44Z
dc.identifier.citationFernández-Díez, C., González-Rojo, S., Lombó, M., & Herráez, M. P. (2016). Impact of sperm DNA damage and oocyte-repairing capacity on trout development. Reproduction, 152(1), 57-67. https://doi.org/10.1530/REP-16-0077es_ES
dc.identifier.issn1470-1626
dc.identifier.otherhttps://rep.bioscientifica.com/view/journals/rep/152/1/57.xmles_ES
dc.identifier.urihttps://hdl.handle.net/10612/18707
dc.descriptionPalabras clave extraídas del títuloes_ES
dc.description.abstract[EN] Zygotic repair of paternal DNA is essential during embryo development. In spite of the interest devoted to sperm DNA damage, its combined effect with defect-repairing oocytes has not been analyzed. Modification of the breeding season is a common practice in aquaculture. This practice reduces developmental success and could affect the both factors: sperm DNA integrity and oocyte repair capacity. To evaluate the maternal role, we analyzed the progeny outcome after fertilizing in-season trout oocytes with untreated and with UV-irradiated sperm. We also analyzed the offspring obtained out of season with untreated sperm. The analysis of the number of lesions in 4 sperm nuclear genes revealed an increase of 1.22–11.18 lesions/10 kb in out-of-season sperm, similar to that obtained after sperm UV irradiation (400 µW/cm25 min). Gene expression showed in out-of-season oocytes the overexpression of repair genes (ogg1, ung, lig3, rad1) and downregulation of tp53, indicating an enhanced repairing activity and reduced capacity to arrest development upon damage. The analysis of the progeny in out-of-season embryos revealed a similar profile tolerant to DNA damage, leading to a much lower apoptotic activity at organogenesis, lower hatching rates and increased rate of malformations. The effects were milder in descendants from in-season-irradiated sperm, showing an enhanced repairing activity at epibolia. Results point out the importance of the repairing machinery provided by the oocyte and show how susceptible it is to environmental changes. Transcripts related to DNA damage signalization and repair could be used as markers of oocyte qualityes_ES
dc.languageenges_ES
dc.publisherSociety for Reproduction and Fertilityes_ES
dc.subjectBiologíaes_ES
dc.subject.otherSpermes_ES
dc.subject.otherDNA damagees_ES
dc.subject.otherOocyte repairinges_ES
dc.subject.otherTrout developmentes_ES
dc.titleImpact of sperm DNA damage and oocyte-repairing capacity on trout developmentes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1530/REP-16-0077
dc.description.peerreviewedSIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/ Plan Nacional de I+D+i/ AGL2011-27787/Análisis de la cromatina espermática de peces y sus aportaciones al control del desarrollo y la calidad de la descendenciaes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.essn1741-7899
dc.journal.titleReproductiones_ES
dc.volume.number152es_ES
dc.issue.number1es_ES
dc.page.initial57es_ES
dc.page.final67es_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES
dc.subject.unesco2407 Biología Celulares_ES
dc.subject.unesco3104.11 Reproducciónes_ES
dc.subject.unesco2401.07 Embriología Animales_ES
dc.description.projectThis work was supported by the Spanish Ministry of Economy and Competitiveness (project AGL2011-27787)es_ES


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