Mostrar el registro sencillo del ítem
dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Fernández Riesco, Marta | |
dc.contributor.author | García Valcarce, David | |
dc.contributor.author | Alfonso Cendón, Javier | |
dc.contributor.author | Herráez Ortega, María Paz | |
dc.contributor.author | Robles Rodríguez, Vanesa | |
dc.contributor.other | Biologia Celular | es_ES |
dc.date | 2014 | |
dc.date.accessioned | 2024-03-22T09:56:28Z | |
dc.date.available | 2024-03-22T09:56:28Z | |
dc.identifier.citation | Riesco, M. F., Valcarce, D. G., Alfonso, J., Herráez, M. P., & Robles, V. (2014). In vitro generation of zebrafish PGC-like cells. Biology of Reproduction, 91(5), Article e114 https://doi.org/10.1095/BIOLREPROD.114.121491 | es_ES |
dc.identifier.issn | 0006-3363 | |
dc.identifier.other | https://academic.oup.com/biolreprod/article/91/5/114,%201-11/2434338 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19309 | |
dc.description.abstract | [EN] The possibility of generating primordial germ cells (PGCs) in vitro from noncommitted embryonic cells represents an extremely useful tool in current research. Primordial germ cell in vitro differentiation has been successfully reported in mammals. However, contrary to fish, PGC specification in mammals is an inductive mechanism. This study is the first to date to describe a rapid method for PGC in vitro differentiation in teleosts. Primordial germ cell-like cells were characterized by several lines of evidence, including gene expression, cell complexity, size, and image analysis for the quantification of fluorescence under vasa promoter. Moreover, differentiated cells were able to colonize the genital ridge after transplantation. Differentiation treatments increased the number of PGCs in culture, causing differentiation of cells rather than inducing their proliferation. These results open up the possibility of differentiating genetically modified embryonic cells to PGC-like cells to ensure their transmission to the progeny and could be crucial for an in-depth understanding of germline differentiation in teleosts | es_ES |
dc.language | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.subject | Biología | es_ES |
dc.subject | Biotecnología | es_ES |
dc.subject.other | Embryonic cell differentiation | es_ES |
dc.subject.other | Gamete biology | es_ES |
dc.subject.other | Primordial germ cells | es_ES |
dc.subject.other | Zebrafish | es_ES |
dc.title | In Vitro Generation of Zebrafish PGC-Like Cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1095/BIOLREPROD.114.121491 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2009-06994/ES/Caracterización, Cultivo y Criopreservación De Pgcs y Sscs De Peces | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN/Programa Nacional de Contratación e Incorporación/RYC-2008-02339 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1529-7268 | |
dc.journal.title | Biology of Reproduction | es_ES |
dc.volume.number | 91 | es_ES |
dc.issue.number | 5 | es_ES |
dc.page.initial | 114 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2407 Biología Celular | es_ES |
dc.subject.unesco | 3104.11 Reproducción | es_ES |
dc.subject.unesco | 2401.07 Embriología Animal | es_ES |
dc.subject.unesco | 2407.01 Cultivo Celular | es_ES |
dc.description.project | Supported by Fundación Ramón Areces and Ministerio de Ciencia e Innovación grant MICINN AGL2009-06994, Ramón y Cajal Program grant RYC-2008-02339, Junta de Castilla y León grants E-24-2009-0036681 and EDU/346/2013, and Fondo Social Europeo. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript | es_ES |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
Artículos [5134]