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dc.contributorFacultad de Ciencias de la Saludes_ES
dc.contributor.authorIglesias García, Graciela
dc.contributor.authorDíaz Rodríguez, Ángel
dc.contributor.authorDíaz Fernández, Beatriz
dc.contributor.authorCuello Estrada, Carmela
dc.contributor.authorGarcía Ferreiro, Tania
dc.contributor.authorCrespo García, Noelia
dc.contributor.authorSeco Calvo, Jesús Ángel 
dc.contributor.otherFisioterapiaes_ES
dc.date2022
dc.date.accessioned2024-04-05T07:51:09Z
dc.date.available2024-04-05T07:51:09Z
dc.identifier.citationIglesias García, G., Díaz Rodríguez, Á., Díaz Fernández, B., Cuello Estrada, C., García Ferreiro, T., Crespo García, N., Seco-Calvo, J.(2022) An Evaluation of Serological Tests to Determine Postvaccinal Immunity to SARS-CoV-2 by mRNA Vaccines. Journal of Clinical Medicine 11(24), 7534; https://doi.org/10.3390/jcm11247534es_ES
dc.identifier.otherhttps://www.mdpi.com/2077-0383/11/24/7534es_ES
dc.identifier.urihttps://hdl.handle.net/10612/19415
dc.description.abstract[EN] Background: The duration of the protective efficacy of vaccines against SARS-CoV-2 is unknown. Thus, an evaluation of the clinical performance of available tests is required. Objectives: To evaluate the clinical performance of LFIA immunoassay compared to ELIA and CLIA immunoassays available in Europe for the detection of IgG antibodies generated by mRNA vaccines against SARS-CoV-2. Methods: Two automated immunoassays (the EUROIMMUN anti-SARS-CoV-2 IgG S1 ELISA and the LIAISON de Diasorin anti-SARS-CoV-2 IgG S1/S2 test) and a lateral flow immunoassay (the Livzon LFIA anti-SARS-CoV-2 IgG S test) were tested. We analyzed 300 samples distributed in three groups: 100 subjects aged over 18 years and under 45 years, 100 subjects aged between 45 and 65 years, and 100 subjects aged over 65 years. The samples were collected before vaccination; at 21 days; and then at 1, 2, 3, and 6 months after vaccination. The sensitivity, specificity, positive predictive value, negative predictive value, positive probability quotient, negative probability quotient, and concordance (kappa index) were calculated for each serological test. Results: The maximum sensitivity values for IgG were 98.7%, 98.1%, and 97.8% for the EUROIMMUN ELISA, Abbott CLIA, and Livzon LFIA tests, respectively, and the maximum specificity values for IgG were 99.4%, 99.9%%, and 98.4% for the ELISA, CLIA, and LFIA tests, respectively, at the third month after vaccination, representing a decrease in the antibody levels after the sixth month. The best agreement was observed between the ELISA and CLIA tests at 100% (k = 1.00). The agreement between the ELIA, CLIA, and LFIA tests was 99% (k = 0.964) at the second and third month after vaccination. Seroconversion was faster and more durable in the younger age groups. Conclusion: Our study examined the equivalent and homogeneous clinical performance for IgG of three immunoassays after vaccination and found LFIA to be the most cost-effective, reliable, and accurate for routine use in population seroconversion and seroprevalence studies.es_ES
dc.languageenges_ES
dc.publisherMDPIes_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFisioterapiaes_ES
dc.subject.otherAutomated immunoassayses_ES
dc.subject.otherCOVID-19es_ES
dc.subject.otherLlateral flow immunoassayses_ES
dc.subject.otherPerformancees_ES
dc.subject.otherSARS-CoV-2es_ES
dc.subject.otherELISA immunoassayses_ES
dc.subject.otherCLIA immunoassayses_ES
dc.titleAn Evaluation of Serological Tests to Determine Postvaccinal Immunity to SARS-CoV-2 by mRNA Vaccineses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.3390/JCM11247534
dc.description.peerreviewedSIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.essn2077-0383
dc.journal.titleJournal of Clinical Medicinees_ES
dc.volume.number11es_ES
dc.issue.number24es_ES
dc.page.initial7534es_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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