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Título
Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship with Campylobacter jejuni
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Frontiers in Cellular and Infection Microbiology
Número de la revista
APR
Cita Bibliográfica
Ayllón, N., Jiménez-Marín, Á., Argüello, H., Zaldívar-López, S., Villar, M., Aguilar, C., Moreno, A., De La Fuente, J., & Garrido, J. J. (2017). Comparative proteomics reveals differences in host-pathogen interaction between infectious and commensal relationship with Campylobacter jejuni. Frontiers in Cellular and Infection Microbiology, 7(APR), Article e145 . https://doi.org/10.3389/FCIMB.2017.00145
Editorial
Frontiers Media
Fecha
2017
Resumen
[EN] Campylobacter jejuni is the leading food-borne poisoning in industrialized countries. While the bacteria causes disease in humans, it merely colonizes the gut in poultry or pigs, where seems to establish a commensal relationship. Until now, few studies have been conducted to elucidate the relationship between C. jejuni and its different hosts. In this work, a comparative proteomics approach was used to identify the underlying mechanisms involved in the divergent outcome following C. jejuni infection in human and porcine host. Human (INT-407) and porcine (IPEC-1) intestinal cell lines were infected by C. jejuni for 3 h (T3h) and 24 h (T24h). C. jejuni infection prompted an intense inflammatory response at T3h in human intestinal cells, mainly characterized by expression of proteins involved in cell spreading, cell migration and promotion of reactive oxygen species (ROS). Proteomic analysis evidenced significantly regulated biofunctions in human cells related with engulfment and endocytosis, and supported by canonical pathways associated to infection such as caveolar- and clathrin-mediated endocytosis signaling. In porcine IPEC-1 cells, inflammatory response as well as signaling pathways that control cellular functions such as cell migration, endocytosis and cell cycle progression resulted downregulated. These differences in the host response to infection were supported by the different pattern of adhesion and invasion proteins expressed by C. jejuni in human and porcine cells. No marked differences in expression of virulence factors involved in adaptive response and iron acquisition functions were observed. Therefore, the results of this study suggest that both host and pathogen factors are responsible for commensal or infectious character of C. jejuni in different hosts.
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- info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-54089-R/ES/INTERACCION PATOGENO-HOSPEDADOR EN LA SALMONELOSIS PORCINA. MODULACION DE LA RESPUESTA INMUNE INTESTINAL POR SALMONELLA Y MECANISMOS DE PERSISTENCIA//
- info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/ FPDI-2013-15619 /ES/ FPDI-2013-15619//
- info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ FJCI-2014-22877/ES/FJCI-2014-22877//
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