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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Barasuol, Bibiana Martins | |
dc.contributor.author | Guizzo, João Antônio | |
dc.contributor.author | Fegan, Jamie Elisabeth | |
dc.contributor.author | Martínez Martínez, Sonia | |
dc.contributor.author | Rodríguez Ferri, Elías Fernando | |
dc.contributor.author | Gutiérrez Martín, César Bernardo | |
dc.contributor.author | Kreutz, Luiz Carlos | |
dc.contributor.author | Schryvers, Anthony Bernard | |
dc.contributor.author | Frandoloso, Rafael | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2017 | |
dc.date.accessioned | 2024-04-12T12:34:50Z | |
dc.date.available | 2024-04-12T12:34:50Z | |
dc.identifier.citation | Barasuol, B. M., Guizzo, J. A., Fegan, J. E., Martínez-Martínez, S., Rodríguez-Ferri, E. F., Gutiérrez-Martín, C. B., Kreutz, L. C., Schryvers, A. B., & Frandoloso, R. (2017). New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis. Scientific Reports, 7(1), Article e10377. https://doi.org/10.1038/S41598-017-10627-0 | es_ES |
dc.identifier.other | https://www.nature.com/articles/s41598-017-10627-0 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19723 | |
dc.description | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | es_ES |
dc.description.abstract | [EN] Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated the presence of cross-reactive epitopes on both the N-lobe and C-lobe of variants of transferrin binding protein B (TbpBs) expressed on the surface of 6 virulent serovars of H. parasuis. Antibodies against TbpB-derived antigens were capable of increasing the phagocytic capacity of neutrophils and were also capable of blocking porcine transferrin from binding to TbpB. Surprisingly, none of the pig or mice antisera from animals immunized with TbpB-derived antigens mixed with Montanide IMS 2215 VG PR adjuvant were able to activate the classical complement pathway (CCP). In contrast, antisera from mice immunized with TbpB-derived antigens adjuvanted with Freund's adjuvants or Montanide Gel 01 were able to activate the CCP and kill H. parasuis. Our results demonstrate that the type of adjuvant can modulate the functional response induced by TbpB-derived antigens. Based on these results, we propose that a properly formulated TbpB-based vaccine may elicit a functional protective antibody response with broad cross-reactivity against heterologous strains of H. parasuis. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Nature Research | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Vaccines | es_ES |
dc.subject.other | Glässer Disease | es_ES |
dc.subject.other | Haemophilus parasuis | es_ES |
dc.subject.other | Pig | es_ES |
dc.subject.other | N-lobe | es_ES |
dc.subject.other | C-lobe | es_ES |
dc.subject.other | Protein B | es_ES |
dc.subject.other | TbpB | es_ES |
dc.subject.other | Transferrin | es_ES |
dc.title | New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1038/S41598-017-10627-0 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2011-23195/ES/PROTEINAS RECOMBINANTES DE HAEMOPHILUS PARASUIS CON AFINIDAD POR LA TRANSFERRINA PORCINA. VALORACION DE SU INMUNIDAD PROTECTORA EN LA ENFERMEDAD DE GLASSER// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2045-2322 | |
dc.journal.title | Scientific Reports | es_ES |
dc.volume.number | 7 | es_ES |
dc.issue.number | 1 | es_ES |
dc.page.initial | 10377 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2412.10 Vacunas | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 32 Ciencias Médicas | es_ES |
dc.description.project | This work was supported by the Conselho Nacional de Desenvolvimento Científco e Tecnológico (CNPq, grant 485807/2013-0) and by the Spanish Ministry of Economy and Competitivity (grant AGL2011-23195). B.B. and J.G. were supported by a Master fellowship from the Fundação Universidade de Passo Fundo and from the Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES), respectively. We would also like to thank Dra. Letícia Gressler for technical assistance in the complement system assays. | es_ES |
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