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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Casais, Rosa | |
dc.contributor.author | Granda García, Víctor | |
dc.contributor.author | Balseiro Morales, Ana María | |
dc.contributor.author | Cerro, Ana del | |
dc.contributor.author | Dalton, Kevin P. | |
dc.contributor.author | González, Roxana | |
dc.contributor.author | Bravo, Pablo | |
dc.contributor.author | Prieto, J. M. | |
dc.contributor.author | Montoya, María | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2016 | |
dc.date.accessioned | 2024-04-18T06:11:06Z | |
dc.date.available | 2024-04-18T06:11:06Z | |
dc.identifier.citation | Casais, R., Granda, V., Balseiro, A., Del Cerro, A., Dalton, K. P., González, R., Bravo, P., Prieto, & Montoya, M. (2016). Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection. Parasites and Vectors, 9, Article e435. https://doi.org/10.1186/S13071-016-1717-9 | es_ES |
dc.identifier.other | https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1717-9 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19900 | |
dc.description | © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | es_ES |
dc.description.abstract | [EN]Background: Vaccination is an attractive ecological alternative to the use of acaricides for parasite control. However, effective anti-parasite vaccines against sarcoptic mange have not yet been developed. The purpose of this study was first to identify Sarcoptes scabiei immunodominant antigens and second to evaluate them as vaccine candidates in a rabbit/S. scabiei var. cuniculi model. Methods: The S. scabiei Ssλ15 immunodominant antigen was selected by immunoscreening of a S. scabiei var. hominis cDNA. The full-length cDNA was sequenced and cloned into the pGEX vector and the recombinant protein expressed in BL21 (DE3) cells and purified. A vaccination trial was performed consisting of a test group (n = 8) immunised with recAgs (a mix of two recombinant antigens, Ssλ15 and the previously described Ssλ20ΔB3) and a control group (n = 8) immunised with PBS. All analyses were performed with R Statistical Environment with α set at 0.050. Results: The full-length open reading frame of the 1,821 nt cloned cDNA encodes a 64 kDa polypeptide, the sequence of which had 96 % identity with a hypothetical protein of S. scabiei. Ssλ15 was localised by immunostaining of skin sections in the tegument surrounding the mouthparts and the coxa in the legs of mites. Rabbit immunisation with recAgs induced high levels of specific IgG (P < 0.010) and increased levels of total IgEs. However, no significant clinical protection against S. scabiei challenge was detected. Unexpectedly, the group immunised with the recAgs mix had significantly higher lesion scores (P = 0.050) although lower mean mite densities than those observed in the control group. These results might indicate that the lesions in the recAgs group were due not only to the mites density but also to an exacerbated immunological response after challenge, which is in agreement with the specific high levels of pro-inflammatory cytokines (IL-1 and TNFα) detected after challenge in this group. Conclusions: The selected antigens delivered as recombinant proteins had no clinical protective efficacy against S. scabiei infestation although immunisation reduced mite density. However, these results pave the way for future studies on alternative production systems, adjuvants, delivery methods and combinations of antigens in order to manage stimulation of clinical protective immune responses. | es_ES |
dc.language | eng | es_ES |
dc.publisher | BMC | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Inmunología | es_ES |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Sarcoptic mange | es_ES |
dc.subject.other | Sarcoptes scabiei | es_ES |
dc.subject.other | Immunodominant antigens | es_ES |
dc.subject.other | Vaccine candidates | es_ES |
dc.subject.other | Clinical protection | es_ES |
dc.title | Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1186/s13071-016-1717-9 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2010-22200-C02-01/ES/CARACTERIZACION DE LA RESPUESTA INMUNE INDUCIDA POR CEPAS DEL VIRUS DE LA GRIPE PORCINA CIRCULANTES EN ESPAÑA. DESARROLLO DE VACUNAS BASADAS EN VLPS QUIMERICAS | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1756-3305 | |
dc.journal.title | Parasites & Vectors | es_ES |
dc.volume.number | 9 | es_ES |
dc.page.initial | 435 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3109.03 Inmunología | es_ES |
dc.description.project | This work was partially funded by grant RTA11-00087-00-00 from the Spanish Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Fondo Europeo de Desarrollo Regional (FEDER), AGL2010-22200-C02-01 from Spanish Ministry (MINECO) and the Biotechnology and Biological Sciences Research Council (BBSRC) grant BBS/E/I/00002014. | es_ES |
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