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Título
Aphanomyces euteiches Cell Wall Fractions Containing Novel Glucan-Chitosaccharides Induce Defense Genes and Nuclear Calcium Oscillations in the Plant Host Medicago truncatula
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
PLoS ONE
Número de la revista
9
Datos de la obra
Nars, A., Lafitte, C., Chabaud, M., Drouillard, S., Mélida, H., Danoun, S., Le Costaouëc, T., Rey, T., Benedetti, J., Bulone, V., Barker, D. G., Bono, J.-J., Dumas, B., Jacquet, C., Heux, L., Fliegmann, J., & Bottin, A. (2013). Aphanomyces euteiches Cell Wall Fractions Containing Novel Glucan-Chitosaccharides Induce Defense Genes and Nuclear Calcium Oscillations in the Plant Host Medicago truncatula. PLoS ONE, 8(9). https://doi.org/10.1371/JOURNAL.PONE.0075039
Editor
Public Library of Science
Fecha
2013
Abstract
[EN] N-acetylglucosamine-based saccharides (chitosaccharides) are components of microbial cell walls and act as molecular signals
during host-microbe interactions. In the legume plant Medicago truncatula, the perception of lipochitooligosaccharide signals
produced by symbiotic rhizobia and arbuscular mycorrhizal fungi involves the Nod Factor Perception (NFP) lysin motif receptor-like
protein and leads to the activation of the so-called common symbiotic pathway. In rice and Arabidopsis, lysin motif receptors are
involved in the perception of chitooligosaccharides released by pathogenic fungi, resulting in the activation of plant immunity. Here
we report the structural characterization of atypical chitosaccharides from the oomycete pathogen Aphanomyces euteiches, and
their biological activity on the host Medicago truncatula. Using a combination of biochemical and biophysical approaches, we show
that these chitosaccharides are linked to β-1,6-glucans, and contain a β-(1,3;1,4)-glucan backbone whose β-1,3-linked glucose
units are substituted on their C-6 carbon by either glucose or N-acetylglucosamine residues. This is the first description of this type
of structural motif in eukaryotic cell walls. Glucan-chitosaccharide fractions of A. euteiches induced the expression of defense
marker genes in Medicago truncatula seedlings independently from the presence of a functional Nod Factor Perception protein.
Furthermore, one of the glucan-chitosaccharide fractions elicited calcium oscillations in the nucleus of root cells. In contrast to the
asymmetric oscillatory calcium spiking induced by symbiotic lipochitooligosaccharides, this response depends neither on the Nod
Factor Perception protein nor on the common symbiotic pathway. These findings open new perspectives in oomycete cell wall
biology and elicitor recognition and signaling in legumes.
Materia
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