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Título
Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Molecules
Número de la revista
14
Datos de la obra
Hernández-Altamirano, J. M., Ugidos, I. F., Palazón, J., Bonfill, M., García-Angulo, P., Álvarez Fernández, J., Acebes, J. L., Bye, R., & Encina, A. (2020). Production of encecalin in cell cultures and hairy roots of Helianthella quinquenervis (Hook.) A. Gray. Molecules, 25(14). https://doi.org/10.3390/MOLECULES25143231
Editor
MDPI
Fecha
2020
Resumo
[EN] Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are
used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to
identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their
potential for biotechnological production. Gas chromatography–mass spectrometry (GC-MS) analysis
corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus
and cell suspensions). In addition, hairy roots were obtained through three transformation protocols
(prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4. After three
months, cocultivation achieved the highest percentage of transformation (66%), and a comparable
production (FW) of encecalin (110 g/g) than the sonication assay (120 g/g), both giving far higher
yields than the prick assay (19 g/g). Stable integration of rolC and aux1 genes in the transformed roots
was confirmed by polymerase chain reaction (PCR). Hairy roots from cocultivation (six months-old)
accumulated as much as 1086 g/g (FW) of encecalin, over three times higher than the cell suspension
cultures. The production of encecalin varied with growth kinetics, being higher at the stationary
phase. This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating
the potential for a future biotechnological production of chromenes.
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