Compartir
Título
Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Biologia plantarum
Número de la revista
1
Cita Bibliográfica
Valdés, Galuszka, Fernández, Centeno, & Frébort. (2007). Developmental stage as a possible factor affecting cytokinin content and cytokinin dehydrogenase activity in Pinus sylvestris. Biologia Plantarum, 51(1), 193-197. https://doi.org/10.1007/S10535-007-0039-3
Editorial
Springer Nature
Fecha
2007
ISSN
0006-3134
Resumen
[EN] In the present study cytokinin dehydrogenase (CKX) activity was for the first time found in a conifer species, Pinus
sylvestris. The activities were correlated with the endogenous cytokinin contents. Several enzyme substrates and two
different electron acceptors were used to search for the enzyme activity in the extract from seeds, seedlings and
plantlets. The highest specific activity was found in one-year-old plantlets with isopentenyladenine as the substrate and
2,6-dichlorophenolindophenol as the electron acceptor, at pH 8. An enhancement in the CKX specific activity
corresponded to increasing contents of cytokinins, mainly isopentenyladenine and isopentenyladenosine, indicating that
the enzyme activity is affected by the endogenous supply of cytokinins. CKX affinity for the ribosylated form of
isopentenyladenine was dependent on the developmental stage, being higher in seeds than in seedlings, and not
detectable in plantlets. The results are indicative of the presence of different isoenzymes throughout the development.
Materia
Palabras clave
Peer review
SI
URI
DOI
Aparece en las colecciones
- Artículos [5503]
Ficheros en el ítem
Tamaño:
269.1
xmlui.dri2xhtml.METS-1.0.size-kilobytes
Formato:
Adobe PDF