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    Título
    Evaluation of two antimicrobial packaging films against Escherichia coli O157:H7 strains in vitro and during storage of a spanish ripened sheep cheese (zamorano)
    Autor
    Otero, Verónica
    Becerril González, Raquel
    Santos, Jesús
    Rodríguez Calleja, José MaríaAutoridad BuleriaORCID
    Nerín, Cristina
    García López, María LuisaAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Veterinaria
    Área de conocimiento
    Nutricion y Bromatologia
    Datos de la obra
    Food Control, 42
    Editor
    Elsevier
    Fecha
    2014-02-23
    Abstract
    Entidades financiadoras: Junta de Castilla y León (Ayuda Grupo de Excelencia GR155 y Proyecto de Investigación LE331A12-2). Proyecto de Investigación AGL2011-26118/ALI. The antimicrobial activity of two packaging films (polypropylene –PP- and polyethylene terephthalate –PET-) coated with different concentrations (0, 4, 6 and 8%) of essential oil from Origanum vulgare (OR) and Ethyl Lauroyl Arginate HCl (LAE) was tested against two E. coli O157:H7 strains using in vitro systems and a raw milk sheep cheese model (Zamorano). The influence of the antimicrobials on the sensory attributes of cheese was also evaluated. For both strains, the MBC (minimum bactericidal concentration) values were identical to their respective MIC (minimum inhibitory concentration) values and lower for LAE (25 mg/l) than for OR (200-400 mg/l). PP and PET films coated with OR were tested by a vapour phase assay and the Japanish Standard method (JIS Z 2801:2000). Both films coated with LAE were tested by an agar diffusion method. Overall, in vitro tests were effective against both strains. The inhibitory activity depended on the active compound concentration, the target strain and the packaging material, PET being more effective than PP. For inoculated cheese slices, OR and LAE PP films did not effectively decrease E. coli O157:H7 counts after 7-days cold storage. PET films incorporating 6 and 8 % of OR and LAE significantly (p< 0.05) decreased the numbers of both strains and also did 4% for the reference and wild strain depending on the antimicrobial. LAE PP, OR PET and LAE PET did not significantly (p> 0.05) affect sensorial characteristics of Zamorano cheese. Packaging with PET films coated with  6% LAE concentrations might be useful in reducing E. coli O157:H7 numbers in sheep cheese
    Materia
    Tecnología de los alimentos
    Veterinaria
    Palabras clave
    E. coli O157:H7
    Ethyl Lauroyl Arginate
    LAE
    Antimicrobial
    Queso de oveja
    Essential oils
    Zamora
    Active packaging
    URI
    http://hdl.handle.net/10612/4166
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