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dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorFernández, Nélida
dc.contributor.authorGarcía, Juan J.
dc.contributor.authorDiez Liébana, María José 
dc.contributor.authorTerán Somaza, María Teresa 
dc.contributor.authorSierra Vega, Matilde 
dc.contributor.otherFarmacologiaes_ES
dc.date1993-05-28
dc.date.accessioned2015-09-04T11:40:56Z
dc.date.available2015-09-04T11:40:56Z
dc.date.issued2015-09-04
dc.identifier.citationJournal of Chromatography, 1993, n. 619es_ES
dc.identifier.urihttp://hdl.handle.net/10612/4574
dc.descriptionP. 143-147es_ES
dc.description.abstractA method for the determination of ethynyloestradiol in samples of rabbit plasma containing pentobarbital and heparin, the former used as an anaesthetic and the latter as an anticoagulant, has been developed. Quantification was carried out using a reversed-phase high-performance liquid chromatographic (HPLC) method in isocratic mode at room temperature, with electrochemical detection at an applied potential of + 1 V vs. Ag/AgCI. Under these conditions, the retention time for ethynyloestradiol was ca. 2.9 min , the average recovery from plasma was 74.5%, and the limit of detection was 10 pg, corresponding to a plasma concentration of 50 pg/ml using 1 ml of plasma. Natural oestrogens, oestriol, oestradiol and oestrone showed peaks that did not interfere with ethynyloestradiol, and retention times of ca. 0.8, 2.4 and 3.4 min, respectively.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectEcología. Medio ambientees_ES
dc.subjectFarmacologíaes_ES
dc.subjectVeterinariaes_ES
dc.subject.otherChromatographices_ES
dc.subject.otherEthinylestradioles_ES
dc.subject.otherRabbit plasmaes_ES
dc.titleRapid high-performance liquid chromatographic assay of ethynyloestradiol in rabbit plasmaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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