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Título
Culture of Differentiated Adult Rabbit Auricular Chondrocytes
Autor
Facultad/Centro
Área de conocimiento
Datos de la obra
Anatomia, Histologia, Embryologia, December 2005, Volume 34, Issue Supplement s1
Editor
Asociación Mundial de Anatomistas Veterinarios
Fecha
2005-12-05
Abstract
Chondrocytes dedifferentiate to a fibroblast-like phenotype
on plastic surfaces. Dedifferentiation is reversible if these
cells are then cultured embedded in gels as alginate, agarose
or collagen. Chondrocytes cultured in suspension on a nonadherent
surface are also known to form aggregates of
differentiated cells. The knowledge of chondrocyte behavior
in culture is relevant for tissue engineering purposes. In
this report we describe a simple method to culture differentiated
or redifferentiated rabbit auricular chondrocytes
on plastic surfaces with a stable phenotype. When chondrocyte
aggregates formed in suspension are next seeded on
plastic surfaces, most of them attach to the plastic as round
or polygonal cells, and this morphological differentiation,
confirmed by the presence of type II collagen, is stable for
long culture periods. We also report that the addition of
aggregates to monolayer cultures of dedifferentiated
chondrocytes results in their redifferentiation, as is shown
by their morphological changes and the synthesis of type II
collagen. Therefore, this simple method can be useful for
the study of chondrocyte behavior on plastic surfaces and
for redifferentiating previously proliferated chondrocytes
in tissue engineering techniques. Furthermore, these results
demonstrate that, in addition to culture conditions such as
cell isolation method or cell-density, chondrocyte behavior
on plastic depends on the presence or absence of aggregates
resulting from the dissociation process
Materia
Peer review
SI
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