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    Título
    Altered indirect hemagglutination method for easy serotyping of Haemophilus parasuis
    Autor
    Lorenson, Monique Salete
    Miani, Michela
    Guizzo, João
    Barasoul, B.
    Martínez Martínez, SoniaAutoridad BuleriaORCID
    Rodríguez Ferri, Elías FernandoAutoridad BuleriaORCID
    Gutiérrez Martín, César BernardoAutoridad BuleriaORCID
    Kreutz, Luiz Carlos
    Frandoloso, Rafael
    Facultad/Centro
    Facultad de Veterinaria
    Área de conocimiento
    Sanidad Animal
    Datos de la obra
    Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2017, vol. 69, n. 1
    Editor
    FEPMVZ Editora
    Fecha
    2017-02-23
    Abstract
    Glässer’s disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect hemagglutination (IHA) is indicated as a serotyping method for H. parasuis. In the present study, we describe an additional step that aims to work around a possible obstacle in the original protocol that may compromise the outcome of this assay. We observed that the choice of anticoagulant for blood collection influences and/or impairs spontaneous adsorption of H. parasuis antigens on sheep red blood cells (SRBCs). However, regardless of the anticoagulant used, chemical treatment of SRBCs with tannic acid induces a stable antigen adsorption (sensitization step). The addition of 1% BSA to SRBCs washing buffer and to antisera dilution augments IHA specificity. Tannic acid treated SRBCs combined with thermo-resistant H. parasuis antigens increases the assay resolution. Thus, our results demonstrate an improvement in the technique of H. parasuis serotyping that will prove valuable to understand Glässer’s disease epidemiology and to better characterize serovars involved in outbreaks
    Materia
    Sanidad animal
    Palabras clave
    Haemophilus parasuis
    Tannic acid
    Indirect hemagglutination
    Serotyping
    Diagnosis
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/6455
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