Show simple item record

dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorMartínez, Sonia
dc.contributor.authorFrandoloso, Rafael
dc.contributor.authorRodríguez Ferri, Elías Fernando 
dc.contributor.authorGonzález Zorn, Bruno
dc.contributor.authorGutiérrez Martín, César Bernardo 
dc.contributor.otherSanidad Animales_ES
dc.date2009-07-23
dc.date.accessioned2017-08-01T17:02:27Z
dc.date.available2017-08-01T17:02:27Z
dc.date.issued2017-08-01
dc.identifier.citationFEMS Microbiology Letters, 2010es_ES
dc.identifier.urihttp://hdl.handle.net/10612/6463
dc.description9 p.es_ES
dc.description.abstractHaemophilus parasuis, the etiological agent of Gl¨asser’s disease in pigs, possesses iron acquisition pathways mediated by a surface receptor that specifically bind porcine transferrin. This receptor is composed of transferrin-binding protein A (TbpA) and TbpB. As it has been reported for other gram-negative organisms, H. parasuis TbpA could be useful as a candidate target for H. parasuis vaccination. In this study, a 600-bp tbpA fragment of the gene encoding TbpA from H. parasuis serovar 5, the Nagasaki strain, was amplified by PCR and cloned into a pBAD/ Thio-TOPO expression vector, generating the pBAD-Thio-TbpA-V5-His (TbpAHis) construction. Escherichia coli LMG194-competent cells were transformed with this construction, followed by the induction of protein expression with arabinose. A band (38.5 kDa) corresponding to a 200-amino acid recombinant TbpA (rTbpA) fragment was seen on the sodium dodecyl sulfate polyacrylamide gel electrophoresis and confirmed by immunoblotting. Polyclonal antibodies raised against this fragment were specific for H. parasuis and Actinobacillus pleuropneumoniae, reacted at the cell surface with H. parasuis, and a significant bactericidal activity was also detected. Therefore, this rTbpA fragment induces an immunological response and might be useful as an antigen for vaccination against Gl¨asser’s diseasees_ES
dc.languageenges_ES
dc.publisherOxford Academices_ES
dc.subjectSanidad animales_ES
dc.subjectVeterinariaes_ES
dc.subject.otherGlässer’s diseasees_ES
dc.subject.otherHaemophilus parasuises_ES
dc.titleCharacterization of a recombinant transferrin-binding proteinA (TbpA) fragment fromHaemophilus parasuis serovar 5es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record