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Título
Evaluation of different API systems for identification of porcine Pasteurella multocida isolates
Autor
Facultad/Centro
Área de conocimiento
Datos de la obra
Research in Veterinary Science, 2008
Editor
Elsevier
Fecha
2008-02-22
Abstract
An exhaustive biochemical characterisation of 60 porcine Pasteurella multocida clinical isolates recovered from lesions indicative of
pneumonia, previously confirmed by PCR and all belonging to the capsular serogroup A, was performed by means of four commercial
systems. The API 20NE correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the API 20E
method. The high diversity exhibited by the API 50CHB/E system, with six different patterns, does not advise its use as additional system
for a definitive identification at the species level, but this method could be a potential tool for characterising P. multocida isolates below
this level. The more uniform reactions yielded by the API ZYM test make this system helpful in the confirmatory identification of this
organism. The high variability (20 profiles) obtained when the four systems are taken together also suggests their usefulness for epidemiological
purposes in order to sub-type P. multocida isolates
Materia
Palabras clave
Peer review
SI
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