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dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorMartínez Martínez, Sonia 
dc.contributor.authorFrandoloso, Rafael
dc.contributor.authorRodríguez Ferri, Elías Fernando 
dc.contributor.authorGarcía-Iglesias, María-José
dc.contributor.authorPérez Martínez, Claudia 
dc.contributor.authorÁlvarez Estrada, Álvaro
dc.contributor.authorGutiérrez Martín, César Bernardo 
dc.contributor.otherSanidad Animales_ES
dc.date2016-07-23
dc.date.accessioned2017-09-28T13:39:01Z
dc.date.available2017-09-28T13:39:01Z
dc.date.issued2017-09-28
dc.identifier.citationVeterinary Immunology and Immunopathology, 2016es_ES
dc.identifier.urihttp://hdl.handle.net/10612/6816
dc.description9 p.es_ES
dc.description.abstractThis study aimed to characterize the type of immune response induced by an experimental vaccine based on a mutant Haemophilus parasuis transferrin binding protein (Tbp) B (Y167A) defective in its ability to bind porcine transferrin. Clinical and pathological signs, bacterial clearance, antibody response and the cytokine profile in alveolar macrophages and spleen after the vaccination and challenge of twenty-two colostrum-deprived pigs with 108 CFU of H. parasuis were analysed. Pigs vaccinated with Y167A were compared to those vaccinated with native TbpB (nTbpB), those treated with a commercial bacterin (CB) against Glässer's disease, those unvaccinated challenged (CH) and those unvaccinated unchallenged (UNCH) pigs. The rectal temperatures of Y167A pigs resembled those of UNCH pigs and were significantly lower than those of the nTbpB, CB and CH animals. A major reduction in pathological changes of the challenged pigs was observed in the Y167A group. H. parasuis was cleared from 88.9% of the samples from Y167A pigs versus 60.0% and 55.6% from those of the CB and nTbpB groups, respectively. The antibody response elicited by Y167A by ELISA was notably higher than that observed for nTbpB and CB pigs and was capable of preventing the expression and secretion of IL-8. The expression of IL-4 and IL-5, which were associated with the specific antibody levels, suggests that the main mechanism of protection conferred by Y167A vaccine is based on a strong T-helper 2 responsees_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectSanidad animales_ES
dc.subject.otherHaemophilus parasuises_ES
dc.subject.otherGlässer's diseasees_ES
dc.subject.otherMutant TbpB vaccinees_ES
dc.subject.otherBacteriases_ES
dc.subject.otherAntibodieses_ES
dc.subject.otherCytokineses_ES
dc.titleA vaccine based on a mutant transferrin binding protein B of Haemophilus parasuis induces a strong T-helper 2 response and bacterial clearance after experimental infectiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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