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Título
Molecular analysis of lungs from pigs immunized with a mutant transferrin binding protein B-based vaccine and challenged with Haemophilus parasuis
Autor
Facultad/Centro
Área de conocimiento
Cita Bibliográfica
Comparative Immunology, Microbiology and Infectious Diseases, 2016
Editorial
Elsevier
Fecha
2016-05-06
Resumen
The molecular analysis of pigs vaccinated with a mutant transferrin-binding
protein B (Y167A) from Haemophilus parasuis was compared with that performed for
unvaccinated challenged (UNCH) and unvaccinated unchallenged (UNUN) pigs.
Microarray analysis revealed that UNCH group showed the most distinct expression
profile for immune response genes, mainly for those genes involved in inflammation
or immune cell trafficking. This fact was confirmed by real-time PCR, in which the
greatest level of differential expression from this group were CD14, CD163, IL-8 and
IL-12. In Y167A group, overexpressed genes included MAP3K8, CD14, IL-12 and
CD163. Proteomics revealed that collagen -1 and peroxiredoxins 2 and 6 were
overexpressed in Y167A pigs. Our study reveals new data on genes and proteins
involved in H. parasuis infection and several candidates of resistance to infection that
are induced by Y167A vaccine. The expression of proinflammatory molecules from
Y176A pigs is similar to their expression in UNUN pigs
Materia
Palabras clave
Peer review
SI
URI
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