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Título
Immunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer’s disease in pigs
Autor
Facultad/Centro
Área de conocimiento
Cita Bibliográfica
Veterinary Immunology and Immunopathology, vol. XXX
Editorial
Elsevier
Fecha
2012-11-26
Resumen
An immunoproteomic analysis of the protective response of subunit and commercial vaccines
in colostrumdeprived pigs against Glässer’s disease was carried out. A mixture of
proteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasaki
strain (serovar 5) was inoculated intramuscularly (PAPTM) and intratracheally (PAPTCp),
along with a commercial bacterin. PAPT were separated using 2 dimensional electrophoresis
(2DE) gels and with them, 2DE Western blots were carried out. A total of 17 spots were
identified as positive with sera of pigs from any of the three vaccinated groups, the highest
number of immunoreactive proteins being detected in those having received PAPTCp.
Among them, six proteins (FKBP-type peptidyl-prolyl cis-trans isomerase, neuraminidase
exo- -sialidase, xanthine-guanine phosphoribosyl transferase, CMP-N-acetylneuraminic
acid synthetase, phenylalanyl-tRNA synthetase and glyceraldehyde 3-phosphate dehydrogenase)
were found to be novel immunogens in H. parasuis. These proteins showed a high
potential as candidates in future subunit vaccines against Glässer’s disease. The three experimental
groups developed specific systemic total IgG (IgGt), IgG1, IgG2 and IgM antibodies
after immunizations. In addition, those receiving PAPTCp yielded a serum IgA response.
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URI
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