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    Título
    Immunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer’s disease in pigs
    Autor
    Martínez Martínez, SoniaAutoridad BuleriaORCID
    Frandoloso, Rafael
    Rodríguez Ferri, Elías FernandoAutoridad BuleriaORCID
    Gil García, María Concepción
    Hernández Haro, Carolina
    Yubero-Delgado, Sheila
    Gutiérrez Martín, César BernardoAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Veterinaria
    Área de conocimiento
    Sanidad Animal
    Datos de la obra
    Veterinary Immunology and Immunopathology, vol. XXX
    Editor
    Elsevier
    Fecha
    2012-11-26
    Abstract
    An immunoproteomic analysis of the protective response of subunit and commercial vaccines in colostrumdeprived pigs against Glässer’s disease was carried out. A mixture of proteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasaki strain (serovar 5) was inoculated intramuscularly (PAPTM) and intratracheally (PAPTCp), along with a commercial bacterin. PAPT were separated using 2 dimensional electrophoresis (2DE) gels and with them, 2DE Western blots were carried out. A total of 17 spots were identified as positive with sera of pigs from any of the three vaccinated groups, the highest number of immunoreactive proteins being detected in those having received PAPTCp. Among them, six proteins (FKBP-type peptidyl-prolyl cis-trans isomerase, neuraminidase exo- -sialidase, xanthine-guanine phosphoribosyl transferase, CMP-N-acetylneuraminic acid synthetase, phenylalanyl-tRNA synthetase and glyceraldehyde 3-phosphate dehydrogenase) were found to be novel immunogens in H. parasuis. These proteins showed a high potential as candidates in future subunit vaccines against Glässer’s disease. The three experimental groups developed specific systemic total IgG (IgGt), IgG1, IgG2 and IgM antibodies after immunizations. In addition, those receiving PAPTCp yielded a serum IgA response.
    Materia
    Sanidad animal
    Veterinaria
    Palabras clave
    Glässer’s disease
    Haemophilus parasuis
    Antibody response
    Pig
    Immunoproteomics
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/6870
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