• español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
Consorcio BUCLE Recolector
  • Contact Us
  • Send Feedback
  • Enlaces y accesos
    • Derechos de autor
    • Políticas
    • Guía de autoarchivo
    • FAQ y ayuda
    • La ULE y el Acceso Abierto
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Repositorio
    Institucional
    Abierto

    Consorcio BUCLE Recolector

    Browse

    All of BULERIACommunities and CollectionsAuthorsDirectoresTitlesSubjectsFacultad/CentroÁrea de conocimientoFecha de creación/publicaciónTitulaciónThis CollectionAuthorsDirectoresTitlesSubjectsFacultad/CentroÁrea de conocimientoFecha de creación/publicaciónTitulación

    My Account

    Login

    Statistics

    View Usage Statistics

    Otros enlaces

    View Item 
    •   BULERIA Home
    • Scientific Production
    • Untitled
    • View Item
    •   BULERIA Home
    • Scientific Production
    • Untitled
    • View Item

    Compartir

    Export

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • rdf
    • mods
    • mets
    • didl
    • premis

    Citas

    Título
    Antibody response of brown trout Salmo trutta injected with pathogenic Saprolegnia parasitica antigenic extracts
    Autor
    Fregeneda Grandes, Juan MiguelAutoridad BuleriaORCID
    Rodríguez Cadenas, Fausto
    Carbajal González, María Teresa
    Aller Gancedo, José MiguelAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Veterinaria
    Área de conocimiento
    Sanidad Animal
    Datos de la obra
    Diseases of aquatic organisms, 2007, vol. 74, n. 2
    Editor
    Inter-Research
    Fecha
    2007-02-28
    Abstract
    Brown trout Salmo trutta injected with antigenic extracts from a pathogenic isolate of Saprolegnia parasitica developed specific antibodies that were detected by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF) and Western blotting (WB), but not by immunodiffusion (ID). Three groups of five 2 yr old brown trout were injected intraperitoneally with 3 different antigenic extracts: small hyphal fragments (HF) and soluble extracts from sonicated mycelia grown in medium with or without β-sytosterol (SEB and SE, respectively). In the 2 groups injected with SE and SEB, antibodies were found in 66.7% of the serum samples by ELISA, 54.5% by IF and 48.5% by WB. In the group injected with HF, only 1 trout survived the experiment, and in this fish only 1 sample was positive by ELISA. The results obtained by ELISA and IF were similar and show that there is cross-reaction between the antigens used. By WB, the proteins most frequently recognised were 2 proteins of 25 and 29 kDa. No significant differences were found in the groups injected with SE or SEB.
    Materia
    Inmunología
    Sanidad animal
    Veterinaria
    Zoología
    Palabras clave
    Truchas
    Saprolegnia parasítica
    Inmunoglobulinas
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/8019
    Collections
    • Untitled [2723]
    Show full item record
    Files in this item
    Nombre:
    DAO 74, 107-111, 2007 (antibodies against Saprolegnia parasitica).pdf
    Tamaño:
    175.2Kb
    Formato:
    Adobe PDF
    Descripción:
    DAO 74, 107-111, 2007 (antibodies against Saprolegnia parasitica)
    Thumbnail
    FilesOpen