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dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorFregeneda Grandes, Juan Miguel 
dc.contributor.authorRodríguez Cadenas, Fausto
dc.contributor.authorCarbajal González, María Teresa
dc.contributor.authorAller Gancedo, José Miguel
dc.contributor.otherSanidad Animales_ES
dc.date2007-02-28
dc.date.accessioned2018-05-27T20:54:24Z
dc.date.available2018-05-27T20:54:24Z
dc.date.issued2018-05-27
dc.identifier.citationDiseases of aquatic organisms, 2007, vol. 74, n. 2es_ES
dc.identifier.urihttp://hdl.handle.net/10612/8019
dc.descriptionP. 107-111es_ES
dc.description.abstractBrown trout Salmo trutta injected with antigenic extracts from a pathogenic isolate of Saprolegnia parasitica developed specific antibodies that were detected by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF) and Western blotting (WB), but not by immunodiffusion (ID). Three groups of five 2 yr old brown trout were injected intraperitoneally with 3 different antigenic extracts: small hyphal fragments (HF) and soluble extracts from sonicated mycelia grown in medium with or without β-sytosterol (SEB and SE, respectively). In the 2 groups injected with SE and SEB, antibodies were found in 66.7% of the serum samples by ELISA, 54.5% by IF and 48.5% by WB. In the group injected with HF, only 1 trout survived the experiment, and in this fish only 1 sample was positive by ELISA. The results obtained by ELISA and IF were similar and show that there is cross-reaction between the antigens used. By WB, the proteins most frequently recognised were 2 proteins of 25 and 29 kDa. No significant differences were found in the groups injected with SE or SEB.es_ES
dc.languageenges_ES
dc.publisherInter-Researches_ES
dc.subjectInmunologíaes_ES
dc.subjectSanidad animales_ES
dc.subjectVeterinariaes_ES
dc.subjectZoologíaes_ES
dc.subject.otherTruchases_ES
dc.subject.otherSaprolegnia parasíticaes_ES
dc.subject.otherAnticuerposes_ES
dc.titleAntibody response of brown trout Salmo trutta injected with pathogenic Saprolegnia parasitica antigenic extractses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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