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dc.contributorFacultad de Veterinariaes_ES
dc.contributor.authorChitneedi, Praveen K.
dc.contributor.authorSuárez Vega, Aroa
dc.contributor.authorMartínez Valladares, María 
dc.contributor.authorArranz Santos, Juan José 
dc.contributor.authorGutiérrez Gil, Beatriz 
dc.contributor.otherProducción Animales_ES
dc.date2018-04-27
dc.date.accessioned2018-07-02T11:24:39Z
dc.date.available2018-07-02T11:24:39Z
dc.date.issued2018-07-02
dc.identifier.citationVeterinary Research, 2018, vol. 49, n. 39es_ES
dc.identifier.urihttp://hdl.handle.net/10612/8284
dc.description8 p.es_ES
dc.description.abstractThe present study exploited the RNA-seq technology to analyze the transcriptome of target tissues affected by the Teladorsagia circumcincta infection in two groups of adult ewes showing different statuses against gastrointestinal nematode (GIN) infection with the aim of identifying genes linked to GIN infection resistance in sheep. For this, based on the accumulated faecal egg count of 18 adult Churra ewes subjected to a first experimental infection with T. circumcincta, six ewes were classified as resistant and six others as susceptible to the infection. These 12 animals were dewormed and infected again. After humanitarian sacrifice of these 12 animals at day 7 post-infection, RNA samples were obtained from abomasal mucosa and lymph node tissues and RNA-Seq datasets were generated using an Illumina HiSeq 2000 sequencer. The distribution of the genes based on their expression level were very similar among the two different tissues and conditions. The differential expression analysis performed with two software (DESeq and EdgeR) only identified common differentially expressed genes (DEGs), a total of 106, in the lymph node samples which were considered as GIN-activated. The enrichment analysis performed for these GIN-activated genes identified some pathways related to cytokine-mediated immune response and the PPARG signaling pathway as well as disease terms related to inflammation and gastro-intestinal diseases as enriched. A systematic comparison with the results of previous studies confirmed the involvement of genes such as ITLN2, CLAC1 and galectins, in the immune mechanism activated against T. circumcincta in resistant sheep. Financiado por la Junta de Castilla y León (Conserjería de Educación) y Fondo Social Europeo (Ref. LE248U14). Ejecución: Universidad de León. Dpto. Producción Animal. Investigador Principal: Dr. Beatriz Gutierrez Giles_ES
dc.languageenges_ES
dc.publisherSpringer Naturees_ES
dc.subjectVeterinariaes_ES
dc.subject.otherTeladorsagia circumcinctaes_ES
dc.subject.otherGanado ovinoes_ES
dc.titleExploring the mechanisms of resistance to Teladorsagia circumcincta infection in sheep through transcriptome analysis of abomasal mucosa and abomasal lymph nodeses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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