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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Fregeneda Grandes, Juan Miguel | |
dc.contributor.author | Olesen, Niels Jørgen | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2007-02-28 | |
dc.date.accessioned | 2018-11-08T23:50:15Z | |
dc.date.available | 2018-11-08T23:50:15Z | |
dc.date.issued | 2018-11-09 | |
dc.identifier.citation | Diseases of aquatic organisms, 2007, vol. 74, n. 2 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10612/9024 | |
dc.description | P. 151-158 | es_ES |
dc.description.abstract | Three serological tests, enzyme linked immunosorbent assay (ELISA), 50% plaque neutralisation test (50%PNT) and Western blotting (WB), were used to detect antibodies against viral haemorrhagic septicaemia virus (VHSV) in 50 rainbow trout broodstock from a rainbow trout farm endemically infected with VHS but with no clinical signs of infection. When the sera were examined by 50%PNT using the VHSV reference isolate DK-F1 or the heat attenuated DK-F25 mutant strain, no neutralizing antibodies were found. In contrast, when one of the virus isolates from the farm (homologous virus) was used in the 50%PNT, 90% of the fish were found to be positive. By examining a panel of different VHSV isolates in 50%PNT, it was demonstrated that the virus isolate used as test antigen could significantly affect the sensitivity and titre determination in 50%PNT for detection of rainbow trout antibodies against VHSV. When the sera were examined for the presence of VHSV antibodies by ELISA or WB, 61% were found to be positive. When conducting WB analysis, the viral glycoprotein was the protein most frequently recognized, followed by the viral nucleoprotein. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Inter-Research | es_ES |
dc.subject | Inmunología | es_ES |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject | Zoología | es_ES |
dc.subject.other | Truchas | es_ES |
dc.subject.other | Serología | es_ES |
dc.subject.other | Inmunoglobulinas | es_ES |
dc.title | Detection of rainbow trout antibodies against viral haemorrhagic septicaemia virus (VHSV) by neutralisation test is highly dependent on the virus isolate used | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |
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