2024-03-28T16:00:03Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/108092020-12-10T08:56:11Zcom_10612_17col_10612_18
Facultad de Ciencias Biologicas y Ambientales
Nadri, T.
Towhidi, Armin
Zeinoaldini, Saeeid
Martínez Pastor, Felipe
Mousavi, M.
Noei, R.
Tar, M.
Mohammadi Sangcheshmeh, Abdollah
Biologia Celular
2019-07-15
2019-05-21T11:25:56Z
2019-05-21T11:25:56Z
2019-05-21
Theriogenology, 2019, vol. 133
https://www.sciencedirect.com/science/article/pii/S0093691X19301098#!
http://hdl.handle.net/10612/10809
P. 38-44
This study was designed to compare the effects on goat spermatozoa cryosurvival of nano-lecithin-based (NL), lecithin-based (L) and egg yolk-based (EY) extenders. Ejaculates were collected from four fertile goats using artificial vagina and diluted with nine extenders. NL and L were tested at concentrations 1%, 2%, 3% and 4% (w/v), versus 15% (v/v) egg yolk-based extender. Overall, sperm quality (higher motility, viability and HOST, and lower apoptosis) was higher for NL than for L treatments (P < 0.05 for most cases, except for 1%). NL at 1% and especially at 4% showed lower motility and viability than 2% or 3% NL. NL at 2% achieved a better performance (P < 0.05) than EY for VCL (131.5 ± 1.3 vs. 120.3 ± 1.9 μm/s), VSL (43.9 ± 1.5 vs. 35.8 ± 1.4 μm/s), LIN (35.7 ± 0.6 vs. 29.3 ± 0.8%), WOB (47.0 ± 0.5 vs. 43.9 ± 0.9%) and viability (66.4 ± 1.7 vs. 52.7 ± 1.9%). Late apoptotic spermatozoa were also lower in 2% NL compared to EY (16.0 ± 0.5 vs. 26.3 ± 1.1%, P < 0.001). EY and 2% NL were compared in an IVF trial, with no significant differences in cleavage (68.8 vs. 70.8%) or blastocyst ratios (21.3 vs. 20.8%). In conclusion, using 2% nanolecithin in semen dilution could improve sperm cryosurvival of goat.
SI
eng
Elsevier
Veterinaria
Nanoparticle
Sperm cryopreservation
Extender
Lecithin
Caprine
Lecithin nanoparticles enhance the cryosurvival of caprine sperm
info:eu-repo/semantics/article