2024-03-29T10:49:16Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/102932020-12-10T09:01:46Zcom_10612_17col_10612_18
Biologia Celular
Martínez Pastor, Felipe
Mata Campuzano, María
Álvarez Rodríguez, Manuel
Álvarez García, Mercedes
Anel Rodríguez, Luis
Paz Cabello, Paulino de
2019-04-17
P. 67-78
Flow cytometry has become an important technique in sperm evaluation and is increasingly used both for routine assessment and for research in veterinary science. We have revised the literature, describing fluorescent probes that have been used for analysing spermatozoa by flow cytometry, regarding: viability, acrosomal status, capacitation, mitochondrial status, apoptotic markers, oxidative stress markers, DNA damage, sperm counting and sperm sizing. Details and problems of some techniques are reviewed, with special attention to the occurrence of non‐sperm particles in the samples (‘debris’). New and promising aspects of flow cytometry, such as sperm sorting using viability markers as selection criteria, are highlighted. The relationship between flow cytometry analyses and fertility and their future improvements are considered.
http://hdl.handle.net/10612/10293
spa
John Wiley & Sons
Probes and Techniques for Sperm Evaluation by Flow Cytometry
info:eu-repo/semantics/article
TEXT
BULERIA. Repositorio Institucional de la Universidad de León
Hispana