2024-03-29T06:40:36Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/111152023-02-07T11:16:09Zcom_10612_17col_10612_18
Genetica
Polanco de la Puente, Carlos Gaspar
Sáenz de Miera Carnicer, Luis Enrique
González Cordero, Ana Isabel
García García, Pedro, 1957-
Fratini, Richard
Vaquero Rodrigo, Francisca
Vences Benito, Francisco Javier
Pérez de la Vega, Marcelino
Revista electrónica on line
Usage of high-throughput sequencing approaches allow for the generation and characterization
of reference transcriptome datasets that support gene-based marker discovery,
which in turn can be used to build genetic maps among other purposes. We have obtained a
transcriptome assembly including 49,453 genes for the lentil (Lens culinaris Medik.) cultivar
Alpo using RNAseq methodology. This transcriptome was used as reference to obtain
6,306 quality polymorphic markers (SNPs and short indels) analyzing genotype data from a
RIL population at F7 generation derived from the interspecific cross between L. culinaris cv.
Alpo and L. odemensis accession ILWL235. L. odemensis is a wild species included in the
secondary gene pool and can be used as a source for gene introgression in lentil breeding
programs. Marker data were used to construct the first genetic interspecific map between
these two species. This linkage map has been used to precisely identify regions of the CDCRedberry
lentil draft genome in which the candidate genes for some qualitative traits (seed
coat spotting pattern, flower color, and stem pigmentation) could be located. The genome
regions corresponding to a significant single quantitative trait locus (QTL) controlling “time
to flowering” located in chromosome 6 and three QTLs regulating seed size and positioned
in chromosomes 1 and 5 (two QTLs) were also identified. Significant QTLs for Ascochyta
blight resistance in lentil were mapped to chromosome 6 in the genome region or close to it
where QTLs for Ascochyta blight resistance have previously been reported.
http://hdl.handle.net/10612/11115
spa
PLoS
2409 Genética
Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil
info:eu-repo/semantics/article
TEXT
BULERIA. Repositorio Institucional de la Universidad de León
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