2024-03-29T15:39:52Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/64702020-12-10T09:01:06Zcom_10612_17col_10612_18
Sanidad Animal
Río, M.L. del
Gutiérrez Martín, César Bernardo
Rodríguez-Barbosa, José I.
Navas, Jesús
Rodríguez Ferri, Elías Fernando
2017-08-02
12 p.
Haemophilus parasuis is the causative agent of Glässer's disease, which is responsible for considerable economic losses in the pigrearing
industry. The aim of the study reported here was the identification, sequencing and molecular characterization of the TonB
region that includes tonB, exbBD, and tbpBA genes in H. parasuis. In addition, two fusion proteins were generated. One of them
(pGEX-6P-1-GST-TbpB) contained the first 501 amino acids of H. parasuis TbpB protein, while the second (pBAD-Thio-TbpBV5-
His) included the first 102 amino acids of H. parasuis TbpB N-terminus domain. A panel of 14 hybridomas secreting monoclonal
antibodies was raised against the two recombinant TbpB fusion proteins. Furthermore, to assess whether the expression of the
H. parasuis ExbB, TbpB, and TbpA proteins was upregulated under conditions of restricted availability of iron, a rabbit polyclonal
antibody against H. parasuis TbpB-His fusion protein was produced. A rabbit polyclonal antibody against serotype 7 of Actinobacillus
pleuropneumoniae ExbB and TbpA proteins was also used for the detection of the homologous proteins in H. parasuis. Overall,
the data indicate that H. parasuis, like other members of the Pasteurellaceae family, possesses the genetic elements of the TonB
region for iron acquisition and the transferrin-binding proteins encoded under this region are upregulated under restricted iron
availability
http://hdl.handle.net/10612/6470
spa
Elsevier
Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis
info:eu-repo/semantics/article
TEXT
BULERIA. Repositorio Institucional de la Universidad de León
Hispana