2024-03-29T05:09:05Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/103172020-12-10T08:58:59Zcom_10612_17col_10612_18
Effects of cryopreservation on head morphometry and its relation with chromatin status in brown bear (Ursus arctos) spermatozoa
Álvarez García, Mercedes
García Macías, Vanesa
Martínez Pastor, Felipe
Martínez, Félix
Barragán Santos, Santiago
Mata Campuzano, María
Garde López-Brea, Julián
Anel Rodríguez, Luis
Paz Cabello, Paulino de
Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
Veterinaria
Bear
Sperm
Cryopreservation
Morphometry
Chromatin
P. 1498-1506
The Cantabrian brown bear (Ursus arctos) is a highly endangered species in Spain and basic studies are necessary in order to bank its germplasm. Sperm heads are mainly made up of chromatin, thus their shape depends partly on chromatin structure. Thawed semen from 10 bears was used to analyze chromatin status by sperm chromatin structure assay (SCSA) and head morphometry by the computer-assisted sperm morphology assessment (CASMA) system. Morphometry was analyzed before and after freezing–thawing in order to evaluate the effects of cryopreservation on sperm heads. Each spermatozoon was measured for four primary parameters (length, L; width, W; area, A; perimeter, P) and derived parameters (ellipticity: L/W, circularity: 4πA/P2, elongation: (L − W)/(L + W), regularity: πLW/4A). All the derived parameters significantly differed between bears. Likewise, cryopreservation affected head morphometry by reducing its size. Clustering based on morphometric parameters separated three subpopulations, one of them being significantly more influenced by the cryopreservation process. We obtained high correlations between head morphometry and SCSA parameters: standard deviation of DNA fragmentation index (SD-DFI) was correlated with perimeter and area (r = 0.75 and r = 0.62, respectively) and DFIm and DFIt (moderate and total DNA fragmentation index) were correlated with perimeter (r = 0.65 and r = 0.67, respectively). Nevertheless, classification of males according to SCSA or head morphometry did not completely agree so the two assays might explain male variability differently. We conclude that cryopreservation affected morphometry at least in a subset of spermatozoa. These results might improve future application of sperm banking techniques in this species.
SI
2008-12
2019-04-16T23:03:58Z
2019-04-16T23:03:58Z
2019-04-17
info:eu-repo/semantics/article
Theriogenology, 2008, vol. 70, n. 9
https://www.sciencedirect.com/science/article/pii/S0093691X08004652#!
http://hdl.handle.net/10612/10317
eng
Elsevier
https://buleria.unileon.es/bitstream/10612/10317/4/21%20Effects%20of%20cryopreservation%20on%20head%20morphometry%20Theriogenology_070_1498_2008.pdf.jpg
Hispana
TEXT
http://rightsstatements.org/vocab/CNE/1.0/
BULERIA. Repositorio Institucional de la Universidad de León
http://hdl.handle.net/10612/10317