2024-03-29T04:55:21Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/103192020-12-10T09:01:29Zcom_10612_17col_10612_18
Sperm Cryopreservation in Brown Bear (Ursus arctos): Preliminary Aspects
Anel Rodríguez, Luis
Álvarez García, Mercedes
Martínez Pastor, Felipe
Gomes Alves, Susana Cláudia
Nicolás, M.
Mata Campuzano, María
Martínez, Félix
Barragán Santos, Santiago
Anel Rodríguez, Enrique
Paz Cabello, Paulino de
Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
Veterinaria
Esperma
Criopreservación
Osos pardos
Ursus arctos
P. 9-17
The development of sperm cryopreservation procedures in brown bear is the basis for establishing a specific genetic resource bank aimed at the preservation of a Cantabric brown bear population, which is seriously threatened. Several issues complicate the development of these cryopreservation procedures: lack of previous specific studies, a high incidence of urospermia and spermagglutination observed in bear ejaculates. Moreover, the availability of individuals for research from these threatened populations is problematic. In the case of the Cantabric brown bear, we have used males from other populations, but of the same species, as surrogates, to carry out a direct extrapolation of the results. Urospermia – Moreover, 70% of the ejaculates are urine contaminated and spermagglutination have a detrimental effect on post‐thawing cell quality recovery in this species. Considering the high value of these samples (autochthonous population with few individuals), a pre‐selection of the ejaculates is not a viable alternative. Preventive methods reducing the mentioned detrimental effects need to be developed. On the basis of previous data, we can suppose that bear spermatozoa resist freezing injuries well. Nevertheless, because of the scarcity of this information, it is necessary to conduct further research on bear semen freezing under field conditions. Epidydimal spermatozoa can be important for genetic resource banking of threatened populations and thus specific cryobiological protocols need to be assayed. To date, 168 brown bear ejaculates have been frozen by the ITRA‐ULE group at the University of León (Spain) in the development of methodologies for the preservation of brown bear sperm.
SI
2008-10
2019-04-16T23:04:45Z
2019-04-16T23:04:45Z
2019-04-17
info:eu-repo/semantics/article
Reproduction in Domestic Animals, 2008, vol. 43, n. 8
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2008.01248.x
http://hdl.handle.net/10612/10319
eng
John Wiley & Sons
https://buleria.unileon.es/bitstream/10612/10319/4/23%20Sperm%20Cryopreservation%20in%20Brown%20Bear%20ReprodDomestAnim_043_S4_0009_2008.pdf.jpg
Hispana
TEXT
http://rightsstatements.org/vocab/CNE/1.0/
BULERIA. Repositorio Institucional de la Universidad de León
http://hdl.handle.net/10612/10319