2024-03-28T21:21:08Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/106732020-12-10T09:02:10Zcom_10612_17col_10612_18
Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa
Nicolás, M.
Álvarez García, Mercedes
Barragán Santos, Santiago
Martínez Pastor, Felipe
Chamorro Álvarez, César Ángel
Álvarez Rodríguez, Manuel
Paz Cabello, Paulino de
Anel Rodríguez, Luis
Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
Veterinaria
Centrifugation
Density gradient
Cryopreservation
Brown bear
Spermatozoa
P. 1119-1128
Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample.
SI
2012-04-01
2019-05-07T10:11:58Z
2019-05-07T10:11:58Z
2019-05-07
info:eu-repo/semantics/article
Theriogenology, 2012, vol. 77, n. 6
https://www.sciencedirect.com/science/article/pii/S0093691X11005310
http://hdl.handle.net/10612/10673
eng
Elsevier
https://buleria.unileon.es/bitstream/10612/10673/4/61%20Evaluation%20of%20the%20qualitative%20and%20quantitative%20effectiveness%20Theriogenology_077_1119_2012.pdf.jpg
Hispana
TEXT
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BULERIA. Repositorio Institucional de la Universidad de León
http://hdl.handle.net/10612/10673