2024-03-28T19:32:44Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/108092020-12-10T08:56:11Zcom_10612_17col_10612_18
Lecithin nanoparticles enhance the cryosurvival of caprine sperm
Nadri, T.
Towhidi, Armin
Zeinoaldini, Saeeid
Martínez Pastor, Felipe
Mousavi, M.
Noei, R.
Tar, M.
Mohammadi Sangcheshmeh, Abdollah
Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
Veterinaria
Nanoparticle
Sperm cryopreservation
Extender
Lecithin
Caprine
P. 38-44
This study was designed to compare the effects on goat spermatozoa cryosurvival of nano-lecithin-based (NL), lecithin-based (L) and egg yolk-based (EY) extenders. Ejaculates were collected from four fertile goats using artificial vagina and diluted with nine extenders. NL and L were tested at concentrations 1%, 2%, 3% and 4% (w/v), versus 15% (v/v) egg yolk-based extender. Overall, sperm quality (higher motility, viability and HOST, and lower apoptosis) was higher for NL than for L treatments (P < 0.05 for most cases, except for 1%). NL at 1% and especially at 4% showed lower motility and viability than 2% or 3% NL. NL at 2% achieved a better performance (P < 0.05) than EY for VCL (131.5 ± 1.3 vs. 120.3 ± 1.9 μm/s), VSL (43.9 ± 1.5 vs. 35.8 ± 1.4 μm/s), LIN (35.7 ± 0.6 vs. 29.3 ± 0.8%), WOB (47.0 ± 0.5 vs. 43.9 ± 0.9%) and viability (66.4 ± 1.7 vs. 52.7 ± 1.9%). Late apoptotic spermatozoa were also lower in 2% NL compared to EY (16.0 ± 0.5 vs. 26.3 ± 1.1%, P < 0.001). EY and 2% NL were compared in an IVF trial, with no significant differences in cleavage (68.8 vs. 70.8%) or blastocyst ratios (21.3 vs. 20.8%). In conclusion, using 2% nanolecithin in semen dilution could improve sperm cryosurvival of goat.
SI
2019-07-15
2019-05-21T11:25:56Z
2019-05-21T11:25:56Z
2019-05-21
info:eu-repo/semantics/article
Theriogenology, 2019, vol. 133
https://www.sciencedirect.com/science/article/pii/S0093691X19301098#!
http://hdl.handle.net/10612/10809
eng
Elsevier
https://buleria.unileon.es/bitstream/10612/10809/4/103%20Lecithin%20nanoparticles%20Theriogenology_Nadri_2019.pdf.jpg
Hispana
TEXT
http://rightsstatements.org/vocab/CNE/1.0/
BULERIA. Repositorio Institucional de la Universidad de León
http://hdl.handle.net/10612/10809