2024-03-28T09:07:01Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/158432023-03-22T01:00:21Zcom_10612_17col_10612_18
Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®
Salman, Amer
Fernández-Alegre, Estela
Francisco-Vázquez, Rubén
Gómez-Martín, Rubén
Fernández-Fernández, Alejandro
Areán-Dablanca, Héctor
Domínguez Fernández de Tejerina, Juan Carlos
González Montaña, José Ramiro
Caamaño Gualdoni, José Néstor
Martínez Pastor, Felipe
Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
Biología
Bull
OPTIXcell
Equilibration time
Sperm cryopreservation
Sperm quality
Fertility
[EN] Semen cryopreservation in bovine livestock is well established, but logistics often require deviations from standard protocols. Extending the equilibration time to the following day is convenient in many situations. To improve our knowledge of the effects of this modification, we studied the post-thawing and post-incubation (4 h, 38 °C) sperm quality after freezing with 4 or 24-h extension in the OPTIXcell extender by using an ample panel of analyses: CASA for motility; flow cytometry for viability, physiology, oxidative stress, and chromatin parameters (DNA fragmentation, chromatin compaction, and thiol groups status); and spectrometry for malondialdehyde production. Semen was obtained from 12 Holstein bulls. The 24-h equilibration time showed few significant effects, with only a tiny decrease in progressive motility and a positive impact on chromatin structure. The incubation removed some of these effects, with the pattern for chromatin compaction remaining the same. No detrimental oxidative stress or increase in apoptotic or capacitation markers was detected. Additionally, the individual bull interacted with the effects of the incubation and the equilibration, especially regarding the chromatin status. Whereas this interaction did not critically affect sperm quality, it could be relevant in practice. Bull fertility as non-return rates (NRR56) was associated with some sperm parameters (especially with an improved chromatin structure) but not in the 4-h post-thawing analysis. Our study supports that extending the equilibration time by at least 24-h is feasible for bull semen freezing with the OPTIXcell extender.
SI
2023
2023-03-21T13:04:42Z
2023-03-21T13:04:42Z
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Salman, A., Fernández-Alegre, E., Francisco-Vázquez, R., Gómez-Martín, R., Fernández-Fernández, A., Areán-Dablanca, H., Domínguez, J. C., González-Montaña, J. R., Caamaño, J. N., & Martínez-Pastor, F. (2023). Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®. Animal Reproduction Science, 250(107209), 107209. https://doi.org/10.1016/j.anireprosci.2023.107209
0378-4320
http://hdl.handle.net/10612/15843
10.1016/j.anireprosci.2023.107209
eng
info:eu-repo/grantAgreement/MIMECO/AGL2016–81890-REDT
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Elsevier
https://buleria.unileon.es/bitstream/10612/15843/5/Extension_Equilibration_Period%20_24%20h_Maintains.pdf.jpg
Hispana
TEXT
http://creativecommons.org/licenses/by/4.0/
BULERIA. Repositorio Institucional de la Universidad de León
http://hdl.handle.net/10612/15843