2024-03-29T08:28:00Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/153862023-01-17T11:26:51Zcom_10612_17col_10612_18
Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer ( Cervus elaphus ) from northern Spain: Microscopy study and molecular analysis
González, Sara
Río González, Mª Luisa del
Díez Baños, Natividad
Hidalgo Argüello, María del Rosario
Martínez, Angelica
Sanidad Animal
Sanidad animal
[EN] Hypoderma spp. larvae were observed subcutaneously in the dorsal and lumbar regions of red deer (Cervus elaphus) hunted in the province of León (northwestern Spain) causing a myiasis. They were removed and initially classified by their size, shape, color, and location under the skin into the three larval stages that parasitize these animals. The morphological characteristics of the first and second-instar are described and from the features of the third-instar the species was identified as Hypoderma actaeon. To accurately identify this species, five isolates of genomic DNA from the third-instar, two from the second-instar and two from first-instar of H. actaeon were analyzed by PCR analysis of the COI region of mt-DNA. The results confirmed that the examined samples exactly matched with H. actaeon. This study has shown the morphological identification of the three larval stages of H. actaeon and, for the first time, the first and second-instar larvae have been molecularly characterized. Finally, identification of only H. actaeon suggests that this species is the only affecting red deer in the Iberian Peninsula.
2022-12-21
2022-12-21
info:eu-repo/semantics/article
González, S., Del Rio, M. L., Diez, M. N., Hidalgo, M. d. R., & Martínez, A. (2022). Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer (Cervus elaphus) from northern Spain: Microscopy study and molecular analysis. Microscopy Research and Technique. https://doi.org/10.1002/JEMT.24247
1059-910X
http://hdl.handle.net/10612/15386
10.1002/jemt.24247
1097-0029
info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/PI16/00002/ES/HVEM/BTLA/CD160 y PD-L1/PD1 como dianas inmunoterapéuticas en el tratamiento de tumores hematopoyéticos
info:eu-repo/grantAgreement/Ministerio de Ciencia y Tecnología/2004-02580-C03-03/BOS
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Wiley-Blackwell