2024-03-28T20:26:41Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/158442023-03-22T01:00:47Zcom_10612_17col_10612_18
Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells
Fernández-Gómez, Paula
Cobo Díaz, José Francisco
Oliveira, Marcia
González Raurich, María Montserrat
Álvarez Ordóñez, Avelino
Prieto Maradona, Miguel
Walsh, James L.
Sivertsvik, Morten
Noriega Fernández, Estefanía
López Fernández, María Mercedes
Tecnologia de los Alimentos
Tecnología de los alimentos
[EN] Plasma-Activated Water (PAW) was generated from tap water using a surface dielectric barrier discharge at different discharge power (26 and 36 W) and activation time (5 and 30 min). The inactivation of a three-strain Listeria monocytogenes cocktail in planktonic and biofilm state was evaluated. PAW generated at 36 W-30 min showed the lowest pH and the highest hydrogen peroxide, nitrates, nitrites contents and effectiveness against cells on planktonic state, resulting in 4.6 log reductions after a 15-min treatment. Although the antimicrobial activity in biofilms formed on stainless steel and on polystyrene was lower, increasing the exposure time to 30 min allowed an inactivation >4.5 log cycles. The mechanisms of action of PAW were investigated using chemical solutions that mimic its physico-chemical characteristics and also RNA-seq analysis. The main transcriptomic changes affected carbon metabolism, virulence and general stress response genes, with several overexpressed genes belonging to the cobalamin-dependent gene cluster.
2023-03-21
2023-03-21
info:eu-repo/semantics/article
Fernández-Gómez, P., Cobo-Díaz, J. F., Oliveira, M., González-Raurich, M., Alvarez-Ordóñez, A., Prieto, M., Walsh, J. L., Sivertsvik, M., Noriega-Fernández, E., & López, M. (2023). Susceptibility and transcriptomic response to plasma-activated water of Listeria monocytogenes planktonic and sessile cells. Food Microbiology, 113(104252), 104252. https://doi.org/10.1016/j.fm.2023.104252
0740-0020
http://hdl.handle.net/10612/15844
10.1016/j.fm.2023.104252
info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-113658RB-C22/ES/NUEVAS ESTRATEGIAS BASADAS EN LA TECNOLOGIA DE PLASMA PARA REDUCIR LA FORMACION DE BIOFILMS EN LA INDUSTRIA LACTEA
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Elsevier