2024-03-29T00:49:46Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/80192020-12-10T09:02:03Zcom_10612_17col_10612_18
00925njm 22002777a 4500
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Fregeneda Grandes, Juan Miguel
author
Rodríguez Cadenas, Fausto
author
Carbajal González, María Teresa
author
Aller Gancedo, José Miguel
author
2018-05-27
Brown trout Salmo trutta injected with antigenic extracts from a pathogenic isolate of
Saprolegnia parasitica developed specific antibodies that were detected by enzyme-linked immunosorbent
assay (ELISA), immunofluorescence (IF) and Western blotting (WB), but not by immunodiffusion
(ID). Three groups of five 2 yr old brown trout were injected intraperitoneally with 3 different
antigenic extracts: small hyphal fragments (HF) and soluble extracts from sonicated mycelia
grown in medium with or without β-sytosterol (SEB and SE, respectively). In the 2 groups injected
with SE and SEB, antibodies were found in 66.7% of the serum samples by ELISA, 54.5% by IF and
48.5% by WB. In the group injected with HF, only 1 trout survived the experiment, and in this fish
only 1 sample was positive by ELISA. The results obtained by ELISA and IF were similar and show
that there is cross-reaction between the antigens used. By WB, the proteins most frequently recognised
were 2 proteins of 25 and 29 kDa. No significant differences were found in the groups injected
with SE or SEB.
Diseases of aquatic organisms, 2007, vol. 74, n. 2
http://hdl.handle.net/10612/8019
Inmunología
Sanidad animal
Veterinaria
Zoología
Antibody response of brown trout Salmo trutta injected with pathogenic Saprolegnia parasitica antigenic extracts