2024-03-28T11:09:10Zhttp://buleria.unileon.es/oai/requestoai:buleria.unileon.es:10612/106662020-12-10T09:02:32Zcom_10612_17col_10612_18
BULERIA. Repositorio Institucional de la Universidad de León
author
Álvarez Rodríguez, Manuel
author
Álvarez García, Mercedes
author
Anel López, Luis
author
Guerra, Camino
author
Chamorro Álvarez, César Ángel
author
Anel Rodríguez, Luis
author
Paz Cabello, Paulino de
author
Martínez Pastor, Felipe
other
Biologia Celular
2019-05-03T12:27:51Z
2019-05-03T12:27:51Z
2019-05-03
Animal Reproduction Science, 2018, vol. 198
https://www.sciencedirect.com/science/article/pii/S0378432018306067#!
http://hdl.handle.net/10612/10666
Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at −20 °C/min). Sperm quality was assessed after recovery and post-thawing (motility by CASA, HOS test, abnormal forms, cytoplasmic droplets, and viability and acrosomal damage by flow cytometry). The sperm mass pH and osmolality showed a positive correlation with time. Total sperm motility dropped after 2 days PM, with progressivity and sperm velocities remained similar up to 3 days PM. Sperm freezability was acceptable, with the post-thawing HOST, motility, progressivity, VAP, VCL, VSL and BCF negatively correlating with PM time. Overall, chamois epidydimal samples were not adequate for preservation after 6 days PM. Freezability capacity could make these spermatozoa suitable for specific ART even if kept refrigerated for several days PM.
Veterinaria
Effect of length of time post-mortem on quality and freezing capacity of Cantabric chamois (Rupicapra pyrenaica parva) epididymal spermatozoa
info:eu-repo/semantics/article
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URL
https://buleria.unileon.es/bitstream/10612/10666/1/94%20Effect%20of%20length%20of%20time%20post-mortem%20Animal%20Reproduction%20Science%202018.pdf
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94 Effect of length of time post-mortem Animal Reproduction Science 2018.pdf
URL
https://buleria.unileon.es/bitstream/10612/10666/3/94%20Effect%20of%20length%20of%20time%20post-mortem%20Animal%20Reproduction%20Science%202018.pdf.txt
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94 Effect of length of time post-mortem Animal Reproduction Science 2018.pdf.txt